Cytokeratin 6 Antibody (B-7): sc-514520

Cytokeratin 6 Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cytokeratin 6 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Cytokeratin 6 antibody (B-7) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 6 belongs to the cytokeratin family, a diverse group of intermediate filament proteins essential for maintaining epithelial cell structural integrity. Cytokeratin proteins are expressed in pairs within keratinized and non-keratinized epithelial tissues, constituting up to 85% of mature keratinocytes in vertebrate epidermis. Cytokeratin 6 plays a vital role in cellular differentiation and tissue specialization, serving as a marker for tissue differentiation and characterization of malignant tumors. Cytokeratin 6 forms alpha-helical coiled-coil dimers that associate laterally to create 10 nm diameter filaments, providing mechanical resilience to epithelial tissues. Pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha regulate Cytokeratin 6 expression, inducing transcription in epidermal keratinocytes through C/EBP β transcription factor. Multiple isoforms of Cytokeratin 6 (6A-6F) are encoded by highly homologous genes in humans, each showing distinct tissue expression patterns, with Cytokeratin 6A predominating in epithelial tissues. Human Cytokeratin 6A gene is located on chromosome 12q13, and mutations in this gene link to various inheritable hair and skin disorders, underlining Cytokeratin 6′s importance in dermatological health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cytokeratin 6 Antibody (B-7) References:

1. Identification of sporadic mutations in the helix initiation motif of keratin 6 in two pachyonychia congenita patients: further evidence for a mutational hot spot.  |  Lin, MT., et al. 1999. Exp Dermatol. 8: 115-9. PMID: 10232401
2. Monilethrix: mutational hotspot in the helix termination motif of the human hair basic keratin 6.  |  Horev, L., et al. 2000. Hum Hered. 50: 325-30. PMID: 10878479
3. Inflammatory versus proliferative processes in epidermis. Tumor necrosis factor alpha induces K6b keratin synthesis through a transcriptional complex containing NFkappa B and C/EBPbeta.  |  Komine, M., et al. 2000. J Biol Chem. 275: 32077-88. PMID: 10887174
4. Interleukin-1 induces transcription of keratin K6 in human epidermal keratinocytes.  |  Komine, M., et al. 2001. J Invest Dermatol. 116: 330-8. PMID: 11180011
5. Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12.  |  Rosenberg, M., et al. 1991. Cytogenet Cell Genet. 57: 33-8. PMID: 1713141
6. Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms.  |  Takahashi, K., et al. 1995. J Biol Chem. 270: 18581-92. PMID: 7543104
7. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
8. Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients.  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
9. Keratins and the skin.  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
10. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
11. Cytokeratin expression, fibrillar organization, and subtle function in liver cells.  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681
12. A mutational hotspot in the 2B domain of human hair basic keratin 6 (hHb6) in monilethrix patients.  |  Korge, BP., et al. 1998. J Invest Dermatol. 111: 896-9. PMID: 9804356

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 6 Antibody (B-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 6 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 6 Antibody (B-7) Specifications

Alternate Product Name: Keratin 6

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 19-34

Epitope Target: Cytokeratin 6

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 6A, Cytokeratin 6B, Cytokeratin 6C and Cytokeratin 6F

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 6 Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Cytokeratin 6/75 antibody is Cytokeratin 6/75 Antibody (H-6): sc-166074.