Cytokeratin 6/75 Antibody (H-6): sc-166074

Cytokeratin 6/75 Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cytokeratin 6/75 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Cytokeratin 6/75 antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 6/75 is a member of the cytokeratin family, consisting of diverse intermediate filament proteins crucial for maintaining epithelial cell structural integrity. These proteins are expressed in pairs within keratinized and non-keratinized epithelial tissues, constituting up to 85% of mature keratinocytes in vertebrate epidermis. α-helical coiled-coil dimers associate laterally to form 10 nm diameter filaments, providing mechanical support and resilience to epithelial tissues. Cytokeratin 6A is predominantly found in epithelial tissues, highlighting importance in tissue differentiation and specialization. Human Cytokeratin 6A gene is located on chromosome 12q13, and mutations are associated with several inheritable hair and skin disorders. Cytokeratin 75, also known as K6HF, maps to the same genetic locus and plays a significant role in the companion layer of hair follicles, emphasizing importance in both normal physiology and disease states.

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Cytokeratin 6/75 Antibody (H-6) References:

1. Identification of sporadic mutations in the helix initiation motif of keratin 6 in two pachyonychia congenita patients: further evidence for a mutational hot spot.  |  Lin, MT., et al. 1999. Exp Dermatol. 8: 115-9. PMID: 10232401
2. Monilethrix: mutational hotspot in the helix termination motif of the human hair basic keratin 6.  |  Horev, L., et al. 2000. Hum Hered. 50: 325-30. PMID: 10878479
3. Inflammatory versus proliferative processes in epidermis. Tumor necrosis factor alpha induces K6b keratin synthesis through a transcriptional complex containing NFkappa B and C/EBPbeta.  |  Komine, M., et al. 2000. J Biol Chem. 275: 32077-88. PMID: 10887174
4. Interleukin-1 induces transcription of keratin K6 in human epidermal keratinocytes.  |  Komine, M., et al. 2001. J Invest Dermatol. 116: 330-8. PMID: 11180011
5. Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12.  |  Rosenberg, M., et al. 1991. Cytogenet Cell Genet. 57: 33-8. PMID: 1713141
6. Cytokeratin 5 and cytokeratin 6 expressions are unconnected in normal and cancerous tissues and have separate diagnostic implications.  |  Völkel, C., et al. 2022. Virchows Arch. 480: 433-447. PMID: 34559291
7. Cytokeratin 6 identifies basal-like subtypes of pancreatic ductal adenocarcinoma with decreased survival.  |  Lyu, SI., et al. 2023. J Cancer Res Clin Oncol. 149: 7539-7546. PMID: 36971797
8. Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms.  |  Takahashi, K., et al. 1995. J Biol Chem. 270: 18581-92. PMID: 7543104
9. Keratins and the skin.  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
10. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
11. A mutational hotspot in the 2B domain of human hair basic keratin 6 (hHb6) in monilethrix patients.  |  Korge, BP., et al. 1998. J Invest Dermatol. 111: 896-9. PMID: 9804356
12. A novel human type II cytokeratin, K6hf, specifically expressed in the companion layer of the hair follicle.  |  Winter, H., et al. 1998. J Invest Dermatol. 111: 955-62. PMID: 9856802

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 6/75 Antibody (H-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 6/75 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 6/75 Antibody (H-6) Specifications

Alternate Product Name: Keratin 6, Keratin 75

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 147-180

Epitope Target: Cytokeratin 6A

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 6A, Cytokeratin 6B, Cytokeratin 6C and Cytokeratin 75

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 6/75 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin