Cytokeratin 20 Antibody (E-9) recommended for detection of Cytokeratin 20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cytokeratin 20 Antibody (E-9): sc-271183

Cytokeratin 20 Antibody (E-9) is rated 4.6 out of 5 by 7.
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Datasheets

    • Anti-Cytokeratin 20 Antibody (E-9) is a mouse monoclonal IgG1 (lambda light chain) Cytokeratin 20 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 39-74 near the N-terminus of Cytokeratin 20 of human origin
    • Anti-Cytokeratin 20 Antibody (E-9) is recommended for detection of Cytokeratin 20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Cytokeratin 20 Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271183 P
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cytokeratin 20 (E-9): sc-271183.

What application is the blocking peptide sc-271183 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good, specific staining IF staining was performed for Merkel cells in mouse skin. The staining was specific whereas weaker (showing much less CK20+ cells than TROMA-1 staining).
Date published: 2018-02-13
Rated 5 out of 5 by from Positive for Human, Mouse & Rat We received this as a sample. Got this to work on 3 species, mouse, rat and human colon. Very easy and great results. I will be buying the product for years.
Date published: 2017-10-23
Rated 5 out of 5 by from 质量不错 一个月前订购的该产品,发货很快,销售服务也很不错,及时联系送货事宜,抗体效价挺好,还会再选用santa cruz的抗体!
Date published: 2017-08-17
Rated 4 out of 5 by from CK20 antibody in Western Blot Detected CK20 in both mouse and human colorectal cancer cell lines by Western blot.
Date published: 2017-07-24
Rated 5 out of 5 by from Good specificity and signal strength in T84 Good specificity and signal strength in T84 whole cell lysate. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Good cytoplasmic staining with paraffin Good cytoplasmic staining with paraffin-embedded human small intestine tissue -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Worked with fusion protein by WB Worked with fusion protein by WB -SCBT QC
Date published: 2014-06-18
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