Cytokeratin 15 Antibody (LHK15): sc-47697

Cytokeratin 15 Antibody (LHK15) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cytokeratin 15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Cytokeratin 15 antibody (LHK15) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 15 (CK15, K15, K1CO, keratin 15, type I cytoskeletal 15) is an intermediate filament protein that plays a crucial role in maintaining the mechanical integrity of epithelial cells, particularly in the hair follicle. Cytokeratin 15 is predominantly located in the hair-follicle bulge, where Cytokeratin 15 serves as a specific marker for stem cells, highlighting Cytokeratin 15′s importance in hair follicle biology and regeneration. Cytokeratin 15 expression is significant in distinguishing between basal cell carcinoma and trichoepithelioma, as both conditions exhibit overlapping morphological features that can complicate diagnosis. Expression patterns of Cytokeratin 15 and Cytokeratin 19, along with the absence of hair keratins, provide valuable insights into the differentiation status of these tumors, making Cytokeratin 15 monoclonal antibody (LHK15) an essential tool for researchers and clinicians in dermatopathology.

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Cytokeratin 15 Antibody (LHK15) References:

1. Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes.  |  Waseem, A., et al. 1999. J Invest Dermatol. 112: 362-9. PMID: 10084315
2. Expression of the hair stem cell-specific keratin 15 in pilar tumors of the skin.  |  Kanitakis, J., et al. 1999. Eur J Dermatol. 9: 363-5. PMID: 10417438
3. Suppression of keratin 15 expression by transforming growth factor beta in vitro and by cutaneous injury in vivo.  |  Werner, S. and Munz, B. 2000. Exp Cell Res. 254: 80-90. PMID: 10623468
4. Genomic organization and amplification of the human keratin 15 and keratin 19 genes.  |  Whittock, NV., et al. 2000. Biochem Biophys Res Commun. 267: 462-5. PMID: 10623642
5. Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line.  |  Badock, V., et al. 2001. Cell Death Differ. 8: 308-15. PMID: 11319614
6. Cytokeratins as markers of follicular differentiation: an immunohistochemical study of trichoblastoma and basal cell carcinoma.  |  Kurzen, H., et al. 2001. Am J Dermatopathol. 23: 501-9. PMID: 11801790
7. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge.  |  Liu, Y., et al. 2003. J Invest Dermatol. 121: 963-8. PMID: 14708593
8. Stem cell markers (cytokeratin 15, cytokeratin 19 and p63) in in situ and invasive cutaneous epithelial lesions.  |  Abbas, O., et al. 2011. Mod Pathol. 24: 90-7. PMID: 20852595
9. An immunohistochemical comparison of cytokeratin 7, cytokeratin 15, cytokeratin 19, CAM 5.2, carcinoembryonic antigen, and nestin in differentiating porocarcinoma from squamous cell carcinoma.  |  Mahalingam, M., et al. 2012. Hum Pathol. 43: 1265-72. PMID: 22285043
10. Immunohistochemical expression of cytokeratin 15, cytokeratin 19, follistatin, and Bmi-1 in basal cell carcinoma.  |  Kim, HC., et al. 2016. Int J Dermatol. 55: 36-44. PMID: 26739632
11. Cytokeratin 15 is an Effective Indicator for Progression and Malignancy of Esophageal Squamous Cell Carcinomas.  |  Shen, YH., et al. 2016. Asian Pac J Cancer Prev. 17: 4217-4222. PMID: 27797221
12. Lack of specificity of cytokeratin-15 loss in scarring alopecias.  |  Mahalingam, M. 2017. J Am Acad Dermatol. 76: e135-e136. PMID: 28325418

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 15 Antibody (LHK15) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 15 Antibody (LHK15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 15 Antibody (LHK15) Specifications

Alternate Product Name: KRT15

Product Clone Name: LHK15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 439-456

Epitope Target: Cytokeratin 15

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 15 Antibody (LHK15): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin