Cytokeratin 1/10 Antibody (LH1): sc-53251

Cytokeratin 1/10 Antibody (LH1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cytokeratin 1/10 protein of mouse, rat, human, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Cytokeratin 1/10 antibody (LH1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 1 and Cytokeratin 10 are crucial components of the cytoskeleton in epithelial cells, providing structural support and resilience to mechanical stress. These proteins are primarily located in the cytoplasm of keratinocytes, where they form intermediate filaments that contribute to the integrity and barrier function of the skin and other epithelial tissues. The presence of Cytokeratin 1 and 10 is particularly important in the differentiation of keratinocytes, as these proteins are expressed in the upper layers of the epidermis, indicating a transition from basal to differentiated states. Expression patterns of Cytokeratin 1 and 10 serve as markers in the diagnosis of various skin disorders and malignancies, making Cytokeratin 1/10 monoclonal antibody (LH1) an invaluable tool for researchers and clinicians studying epithelial biology and pathology.

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Cytokeratin 1/10 Antibody (LH1) References:

1. Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells.  |  Chuang, NN. and Huang, CC. 2007. Biochem Soc Trans. 35: 1292-4. PMID: 17956333
2. Cytokeratin 10 and cytokeratin 19: predictive markers for poor prognosis in hepatocellular carcinoma patients after curative resection.  |  Yang, XR., et al. 2008. Clin Cancer Res. 14: 3850-9. PMID: 18559605
3. P-cadherin controls the differentiation of oral keratinocytes by regulating cytokeratin 1/10 expression via C/EBP-beta-mediated signaling.  |  Bauer, K., et al. 2012. Differentiation. 84: 345-54. PMID: 23142730
4. The Hsp90 inhibitor 17-AAG represses calcium-induced cytokeratin 1 and 10 expression in HaCaT keratinocytes.  |  Miyoshi, S., et al. 2012. FEBS Open Bio. 2: 47-50. PMID: 23650580
5. Loss of cytokeratin 10 indicates malignant transformation in actinic cheilitis.  |  Garcia, NG., et al. 2016. Clin Oral Investig. 20: 745-52. PMID: 26264639
6. Significance of Cytokeratin-1 Single-Nucleotide Polymorphism and Protein Level in Susceptibility to Vocal Leukoplakia and Laryngeal Squamous Cell Carcinoma.  |  Yang, Y., et al. 2019. ORL J Otorhinolaryngol Relat Spec. 81: 121-129. PMID: 31067553
7. Kallikrein-Mediated Cytokeratin 10 Degradation Is Required for Varicella Zoster Virus Propagation in Skin.  |  Tommasi, C., et al. 2020. J Invest Dermatol. 140: 774-784.e11. PMID: 31626786
8. Cytokeratin 10 (CK10) expression in cancer: A tissue microarray study on 11,021 tumors.  |  Uhlig, R., et al. 2022. Ann Diagn Pathol. 60: 152029. PMID: 36029589
9. Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells.  |  Siew, ZY., et al. 2024. Microbes Infect. 26: 105243. PMID: 38380604
10. Differentiation of eccrine poroma cells to cytokeratin 1- and 10-expressing cells, the intermediate layer cells of eccrine sweat duct, in the tumor cell nests.  |  Ban, M., et al. 1997. J Cutan Pathol. 24: 246-8. PMID: 9138117

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 1/10 Antibody (LH1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 1/10 Antibody (LH1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 1/10 Antibody (LH1) Specifications

Alternate Product Name: KRT1, KRT10

Product Clone Name: LH1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cytokeratin 1 and 10

Epitope Species: human

Product Reactivity/Detection: Cytokeratin 1 and Cytokeratin 10

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 1/10 Antibody (LH1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cytokeratin 10 antibody is Cytokeratin 10 Antibody (RKSE60): sc-23877.