Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cytoglobin CRISPR/Cas9 KO Plasmid (h): sc-402949
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cytoglobin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cytoglobin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cytoglobin Antibody (D-7): sc-365246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cytoglobin CRISPR/Cas9 KO Plasmid (h) | sc-402949 | 20 µg | $397.00 |
Overview
CYGB encodes cytoglobin, a hexacoordinate globin expressed in multiple tissues that binds oxygen and nitric oxide and contributes to cellular redox balance. Cytoglobin is implicated in protection from oxidative and nitrosative stress, influencing hypoxia-responsive signaling, reactive oxygen species handling, and nitric oxide metabolism. Through these processes, CYGB has been connected to regulation of fibroblast activation, vascular function, and cellular adaptation to low-oxygen microenvironments. Altered CYGB expression has been reported in contexts relevant to tumor biology, inflammatory stress, and organ fibrosis, supporting its use as a mechanistic target in pathway and phenotype studies.
cytoglobin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CYGB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CYGB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CYGB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cytoglobin protein expression.
This CRISPR knockout system enables efficient generation of CYGB-deficient cell models for investigation of cytoglobin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CYGB exon(s) critical for cytoglobin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CYGB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cytoglobin CRISPR/Cas9 KO Plasmid (h) and cytoglobin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CYGB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cytoglobin HDR Plasmid (h) and cytoglobin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CYGB homology arms to support homology-directed repair at defined CYGB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.