cyt19 Antibody (F-9): sc-377436
- cyt19 Antibody (F-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 177-375 mapping at the C-terminus of cyt19 of human origin
- Anti-cyt19 Antibody (F-9) is recommended for detection of cyt19 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyt19 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyt19 Antibody (F-9) for WB applications. These reagents are now offered in bundles with cyt19 Antibody (F-9) (see ordering information below).
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SEE ALSO...
cyt19 Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyt19 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). cyt19 Antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyt19, also known as arsenite methyltransferase, plays a crucial role in the metabolism of inorganic arsenic by catalyzing the transfer of a methyl group from S-adenosylmethionine to trivalent arsenicals, resulting in the formation of less toxic methylated metabolites. Specifically, cyt19 methylates arsenite to produce methylarsonate, which is subsequently reduced to methylarsonite; the latter is then converted into dimethylarsinate, a significantly less toxic compound. cyt19 localization in the cytoplasm is vital for efficient interaction with substrates and facilitates the detoxification process. cyt19 is highly expressed in the liver, an organ central to detoxification, and inherited variations in cyt19 may influence individual differences in arsenic metabolism and susceptibility to arsenic-related carcinogenesis in humans.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyt19 Antibody (F-9) References:
- Selenium compounds modulate the activity of recombinant rat AsIII-methyltransferase and the methylation of arsenite by rat and human hepatocytes. | Walton, FS., et al. 2003. Chem Res Toxicol. 16: 261-5. PMID: 12641425
- Endogenous reductants support the catalytic function of recombinant rat cyt19, an arsenic methyltransferase. | Waters, SB., et al. 2004. Chem Res Toxicol. 17: 404-9. PMID: 15025511
- Elucidating the pathway for arsenic methylation. | Thomas, DJ., et al. 2004. Toxicol Appl Pharmacol. 198: 319-26. PMID: 15276411
- A new metabolic pathway of arsenite: arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19. | Hayakawa, T., et al. 2005. Arch Toxicol. 79: 183-91. PMID: 15526190
- Interindividual variation in the metabolism of arsenic in cultured primary human hepatocytes. | Drobná, Z., et al. 2004. Toxicol Appl Pharmacol. 201: 166-77. PMID: 15541756
- Developmentally restricted genetic determinants of human arsenic metabolism: association between urinary methylated arsenic and CYT19 polymorphisms in children. | Meza, MM., et al. 2005. Environ Health Perspect. 113: 775-81. PMID: 15929903
- Human arsenic methyltransferase (AS3MT) pharmacogenetics: gene resequencing and functional genomics studies. | Wood, TC., et al. 2006. J Biol Chem. 281: 7364-73. PMID: 16407288
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyt19 Antibody (F-9) | sc-377436 | 200 µg/ml | $322.00 | |||
cyt19 Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-527602 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyt19 Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-532975 | 200 µg Ab; 20 µg BP | $361.00 | |||
cyt19 Antibody (F-9) AC | sc-377436 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyt19 Antibody (F-9) HRP | sc-377436 HRP | 200 µg/ml | $322.00 | |||
cyt19 Antibody (F-9) FITC | sc-377436 FITC | 200 µg/ml | $336.00 | |||
cyt19 Antibody (F-9) PE | sc-377436 PE | 200 µg/ml | $349.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 488 | sc-377436 AF488 | 200 µg/ml | $364.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 546 | sc-377436 AF546 | 200 µg/ml | $364.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 594 | sc-377436 AF594 | 200 µg/ml | $364.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 647 | sc-377436 AF647 | 200 µg/ml | $364.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 680 | sc-377436 AF680 | 200 µg/ml | $364.00 | |||
cyt19 Antibody (F-9) Alexa Fluor® 790 | sc-377436 AF790 | 200 µg/ml | $364.00 |