CyPA Antibody (6-YD13) recommended for detection of CyPA of mouse, rat and human origin by WB, IP and ELISA

CyPA Antibody (6-YD13): sc-134310

CyPA Antibody (6-YD13) is rated 4.2 out of 5 by 5.
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Datasheets

    • Anti-CyPA Antibody (6-YD13) is a mouse monoclonal IgG2a κ CyPA antibody, cited in 6 publications, provided at 100 µg/ml
    • raised against full length CyPA of human origin
    • recommended for detection of CyPA of mouse, rat and human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CyPA (6-YD13): sc-134310.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
Thank you for your question We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 1 out of 5 by from Not nearly as good as their old rabbit polyclonal! I did an IP to pull down CypA (using abcam's antibody as its better), and then came back with this 6-YD13 mouse monoclonal antibody (right hand blot on image). This is in HEK cells. The antibody band for CypA is very weak signal and the antibody bands for the light chain and heavy chain from the IP beads is very overpowering- I never saw this with the old CypA antibody. I thought that maybe the IP extract is degraded (unlikely though as I've done this lots of times before using my protocol). So I reprobed the same blot with the old C-14 polyclonal rabbit antibody I had left over and the bands for CypA are clear as day . Both antibodies were used at 1:1000 diluted in 5% BSA PBST. I can't increase the amount of 6-YD13 used as it will also increase the signal of the light/heavy chain and will bleach the CypA signal more.
Date published: 2017-07-17
Rated 5 out of 5 by from Strong signal in WB Very clear band in WB at 1:500 dilution. Several publications available for WB and IF in mouse and human samples.
Date published: 2016-12-21
Rated 5 out of 5 by from powerful tool Made outstanding contribution in my work and paper。
Date published: 2016-11-03
Rated 5 out of 5 by from Robust band in western blot analysis of CyPA Robust band in western blot analysis of CyPA expression in K-562 whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Strong band present in western blot using Strong band present in western blot using Jurkat whole cell lysate. -SCBT QC
Date published: 2013-10-04
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