CYP8B1 CRISPR/Cas9 KO Plasmid (h): sc-403383
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP8B1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CYP8B1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYP8B1 Antibody (M15-P3B7): sc-101387
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP8B1 CRISPR/Cas9 KO Plasmid (h) | sc-403383 | 20 µg | $397.00 |
Overview
CYP8B1 encodes a cytochrome P450 enzyme localized to the endoplasmic reticulum that catalyzes 12α-hydroxylation of bile acid intermediates, a key determinant of cholic acid production and the cholic acid/chenodeoxycholic acid ratio. By controlling bile acid composition, CYP8B1 influences enterohepatic signaling through FXR and TGR5, shaping hepatic lipid and glucose metabolism and broader sterol homeostasis. Altered CYP8B1 activity is linked to dysregulated bile acid pools observed in metabolic liver disease, cholestatic states, and conditions affecting cholesterol handling. This makes CYP8B1 a useful node for dissecting bile acid–driven transcriptional programs and crosstalk between hepatocytes and intestinal signaling.
CYP8B1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CYP8B1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CYP8B1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CYP8B1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CYP8B1 protein expression.
This CRISPR knockout system enables efficient generation of CYP8B1-deficient cell models for investigation of CYP8B1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CYP8B1 exon(s) critical for CYP8B1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CYP8B1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CYP8B1 CRISPR/Cas9 KO Plasmid (h) and CYP8B1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CYP8B1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CYP8B1 HDR Plasmid (h) and CYP8B1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CYP8B1 homology arms to support homology-directed repair at defined CYP8B1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.