Cyp4f13 Antibody (G-5): sc-515735

Cyp4f13 Antibody (G-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects CYP4F3 in human, mouse, and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CYP4F3 antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP4F3, also known as cytochrome P450 family 4 subfamily F member 3, plays a crucial role in the metabolism of bioactive lipids, particularly in the degradation of leukotriene B4, a potent inflammatory mediator. This function helps regulate inflammatory responses and maintain homeostasis in various physiological processes. CYP4F3 exists as two alternatively spliced isoforms, CYP4F3A and CYP4F3B, and is encoded by a gene located on human chromosome 19. CYP4F3 consists of 520 amino acids and is part of the larger cytochrome P450 superfamily, which is essential for the oxidative metabolism of various endogenous and exogenous compounds. Understanding CYP4F3′s role in lipid metabolism and inflammation can provide insights into therapeutic targets for inflammatory diseases and related conditions.

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Cyp4f13 Antibody (G-5) References:

1. Expression of the CYP4F3 gene. tissue-specific splicing and alternative promoters generate high and low K(m) forms of leukotriene B(4) omega-hydroxylase.  |  Christmas, P., et al. 1999. J Biol Chem. 274: 21191-9. PMID: 10409674
2. Identification of CYP4F8 in human seminal vesicles as a prominent 19-hydroxylase of prostaglandin endoperoxides.  |  Bylund, J., et al. 2000. J Biol Chem. 275: 21844-9. PMID: 10791960
3. Alternative splicing determines the function of CYP4F3 by switching substrate specificity.  |  Christmas, P., et al. 2001. J Biol Chem. 276: 38166-72. PMID: 11461919
4. Expression of CYP4F8 (prostaglandin H 19-hydroxylase) in human epithelia and prominent induction in epidermis of psoriatic lesions.  |  Stark, K., et al. 2003. Arch Biochem Biophys. 409: 188-96. PMID: 12464258
5. Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants.  |  Nelson, DR., et al. 2004. Pharmacogenetics. 14: 1-18. PMID: 15128046
6. Oxygenation of polyunsaturated long chain fatty acids by recombinant CYP4F8 and CYP4F12 and catalytic importance of Tyr-125 and Gly-328 of CYP4F8.  |  Stark, K., et al. 2005. Arch Biochem Biophys. 441: 174-81. PMID: 16112640
7. Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production.  |  Harmon, SD., et al. 2006. Prostaglandins Leukot Essent Fatty Acids. 75: 169-77. PMID: 16820285
8. Cytochromes P450 from family 4 are the main omega hydroxylating enzymes in humans: CYP4F3B is the prominent player in PUFA metabolism.  |  Fer, M., et al. 2008. J Lipid Res. 49: 2379-89. PMID: 18577768
9. Confirmation of Ath26 locus on chromosome 17 and identification of Cyp4f13 as an atherosclerosis modifying gene.  |  Han, J., et al. 2019. Atherosclerosis. 286: 71-78. PMID: 31102955
10. CYP4F13 is the Major Enzyme for Conversion of alpha-Eleostearic Acid into cis-9, trans-11-Conjugated Linoleic Acid in Mouse Hepatic Microsomes.  |  Wu, Q. and Tsuduki, T. 2020. J Oleo Sci. 69: 1061-1075. PMID: 32879197
11. Full-length transcriptomics study of Ustiloxins-induced hepatocyte injury.  |  Zhang, G., et al. 2024. Toxicon. 238: 107604. PMID: 38181838
12. The cytochrome P450 4 (CYP4) family.  |  Simpson, AE. 1997. Gen Pharmacol. 28: 351-9. PMID: 9068972

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cyp4f13 Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Cyp4f13 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cyp4f13 Antibody (G-5) Specifications

Alternate Product Name: Cyp4f13

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 270-287

Epitope Target: Cyp4f13

Epitope Species: mouse

Product Reactivity/Detection: CYP4F3 of human origin, Cyp4f13 of mouse origin and Cyp4f6 of rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Cyp4f13 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin