CYP4A1/A2/A3 Antibody (clo4): sc-53247

CYP4A1/A2/A3 Antibody (clo4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYP4A1/A2/A3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. CYP4A1/A2/A3 (clo4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP4A enzymes play a crucial role in fatty acid metabolism and bioactive lipid biosynthesis, which are essential for maintaining cellular homeostasis and regulating inflammatory responses. CYP4A1, CYP4A2, and CYP4A3 are involved in omega-hydroxylation of fatty acids, leading to hydroxyeicosatetraenoic acids (HETEs) production, which are key mediators in various physiological processes, including blood pressure regulation and immune response modulation. CYP4A enzymes are highly inducible by peroxisome proliferators, making these proteins significant in pharmacological and toxicological studies. CYP4A1/A2/A3 proteins are expressed in various tissues, including liver, kidney, and brain microvessels, highlighting their importance in both metabolic and neurological functions.

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CYP4A1/A2/A3 Antibody (clo4) References:

1. Molecular basis for the omega-regiospecificity of the CYP4A2 and CYP4A3 fatty acid hydroxylases.  |  Hoch, U., et al. 2000. J Biol Chem. 275: 26952-8. PMID: 10869363
2. CYP4A1 antisense oligonucleotide reduces mesenteric vascular reactivity and blood pressure in SHR.  |  Wang, MH., et al. 2001. Am J Physiol Regul Integr Comp Physiol. 280: R255-61. PMID: 11124159
3. The CYP4A isoforms hydroxylate epoxyeicosatrienoic acids to form high affinity peroxisome proliferator-activated receptor ligands.  |  Cowart, LA., et al. 2002. J Biol Chem. 277: 35105-12. PMID: 12124379
4. Transfection of CYP4A1 cDNA increases vascular reactivity in renal interlobar arteries.  |  Kaide, J., et al. 2003. Am J Physiol Renal Physiol. 284: F51-6. PMID: 12388396
5. Transfection of CYP4A1 cDNA decreases diameter and increases responsiveness of gracilis muscle arterioles to constrictor stimuli.  |  Zhang, F., et al. 2004. Am J Physiol Heart Circ Physiol. 287: H1089-95. PMID: 15130884
6. Expression of CYP4A1 in U251 human glioma cell induces hyperproliferative phenotype in vitro and rapidly growing tumors in vivo.  |  Guo, AM., et al. 2008. J Pharmacol Exp Ther. 327: 10-9. PMID: 18591218
7. Contribution of iNOS/sGC/PKG pathway, COX-2, CYP4A1, and gp91(phox) to the protective effect of 5,14-HEDGE, a 20-HETE mimetic, against vasodilation, hypotension, tachycardia, and inflammation in a rat model of septic shock.  |  Tunctan, B., et al. 2013. Nitric Oxide. 33: 18-41. PMID: 23684565
8. Impaired myogenic response and autoregulation of cerebral blood flow is rescued in CYP4A1 transgenic Dahl salt-sensitive rat.  |  Fan, F., et al. 2015. Am J Physiol Regul Integr Comp Physiol. 308: R379-90. PMID: 25540098
9. The rat clofibrate-inducible CYP4A gene subfamily. I. Complete intron and exon sequence of the CYP4A1 and CYP4A2 genes, unique exon organization, and identification of a conserved 19-bp upstream element.  |  Kimura, S., et al. 1989. DNA. 8: 503-16. PMID: 2766932
10. Identification and characterization of DNA elements implicated in the regulation of CYP4A1 transcription.  |  Aldridge, TC., et al. 1995. Biochem J. 306 (Pt 2): 473-9. PMID: 7887901
11. Cloning, sequencing, and cDNA-directed expression of the rat renal CYP4A2: arachidonic acid omega-hydroxylation and 11,12-epoxidation by CYP4A2 protein.  |  Wang, MH., et al. 1996. Arch Biochem Biophys. 336: 240-50. PMID: 8954571
12. Regulation of CYP4A1 and peroxisome proliferator-activated receptor alpha expression by interleukin-1beta, interleukin-6, and dexamethasone in cultured fetal rat hepatocytes.  |  Parmentier, JH., et al. 1997. Biochem Pharmacol. 54: 889-98. PMID: 9354589

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CYP4A1/A2/A3 Antibody (clo4) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CYP4A1/A2/A3 Antibody (clo4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CYP4A1/A2/A3 Antibody (clo4) Specifications

Alternate Product Name: CYP4A1/A2/A3

Product Clone Name: clo4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: liver cytochrome P450 4A2 and 4A3

Epitope Species: rat

Product Reactivity/Detection: CYP4A1, CYP4A2 and CYP4A3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CYP4A1/A2/A3 Antibody (clo4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CYP4A1 antibody is CYP4A1 Antibody (clo1): sc-53248.