CYP2F1 Antibody (E-10): sc-377499

CYP2F1 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CYP2F1 protein of human origin and CYP2F2 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP2F1 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP2F1, or cytochrome P450 2F1, is a crucial membrane-bound protein predominantly localized to the endoplasmic reticulum membrane, particularly within lung tissue. CYP2F1 plays a vital role in the metabolism of potentially carcinogenic pneumotoxins, which is significant given the increasing concerns about air quality and respiratory health. CYP2F1′s polymorphic nature can influence individual susceptibility to these toxins, as CYP2F1 catalyzes redox reactions that can either detoxify or activate harmful compounds. Specifically, within Clara cells, CYP2F1 converts Skatole (3-methylindole) into 3-methyleneindolenine, an electrophile that can form dangerous protein adducts, thereby disrupting cellular functions. Notably, Skatole has been used as a flavoring agent in cigarettes, highlighting the relevance of CYP2F1 in toxicology and public health. Additionally, CYP2F2 serves as the murine homolog of human CYP2F1, allowing for comparative studies across species.

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CYP2F1 Antibody (E-10) References:

1. Specific dehydrogenation of 3-methylindole and epoxidation of naphthalene by recombinant human CYP2F1 expressed in lymphoblastoid cells.  |  Lanza, DL., et al. 1999. Drug Metab Dispos. 27: 798-803. PMID: 10383923
2. Sp1 and Sp3 regulate basal transcription of the human CYP2F1 gene.  |  Wan, J., et al. 2005. Drug Metab Dispos. 33: 1244-53. PMID: 15860659
3. Cytochrome P450 expression and metabolic activation of cooked food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in MCF10A breast epithelial cells.  |  Thomas, RD., et al. 2006. Chem Biol Interact. 160: 204-16. PMID: 16527260
4. [Study on genetic polymorphisms of CYP2F1 gene in Guangdong population of China].  |  Jiang, JH., et al. 2006. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 23: 383-7. PMID: 16883522
5. Molecular analysis of the CYP2F1 gene: identification of a frequent non-functional allelic variant.  |  Tournel, G., et al. 2007. Mutat Res. 617: 79-89. PMID: 17327131
6. Lithium toxicity and expression of stress-related genes or proteins in A549 cells.  |  Allagui, MS., et al. 2007. Biochim Biophys Acta. 1773: 1107-15. PMID: 17512992
7. Reverse transcriptase-PCR quantification of mRNA levels from cytochrome (CYP)1, CYP2 and CYP3 families in 22 different human tissues.  |  Bièche, I., et al. 2007. Pharmacogenet Genomics. 17: 731-42. PMID: 17700362
8. CYP2F1 genetic polymorphism: identification of interethnic variations.  |  Tournel, G., et al. 2007. Xenobiotica. 37: 1433-8. PMID: 17943660
9. Mechanism-based inactivation of lung-selective cytochrome P450 CYP2F enzymes.  |  Kartha, JS. and Yost, GS. 2008. Drug Metab Dispos. 36: 155-62. PMID: 17962375
10. Human drug metabolism genes in parathion-and estrogen-treated breast cells.  |  Calaf, GM. and Roy, D. 2007. Int J Mol Med. 20: 875-81. PMID: 17982697
11. Localization of CYP2F1 by multipoint linkage analysis and pulsed-field gel electrophoresis.  |  Bale, AE., et al. 1991. Genomics. 10: 284-6. PMID: 2045106
12. Human CYP2A13 and CYP2F1 Mediate Naphthalene Toxicity in the Lung and Nasal Mucosa of CYP2A13/2F1-Humanized Mice.  |  Li, L., et al. 2017. Environ Health Perspect. 125: 067004. PMID: 28599267

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CYP2F1 Antibody (E-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CYP2F1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CYP2F1 Antibody (E-10) Specifications

Alternate Product Name: CYP2F1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 451-491

Epitope Target: CYP2F1

Epitope Species: human

Product Reactivity/Detection: CYP2F1 of human origin and CYP2F2 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CYP2F1 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin