CYP2B1/2B2 Antibody (h7): sc-53244

CYP2B1/2B2 Antibody (h7) is a mouse monoclonal IgG1 antibody that detects CYP2B1/2B2 in mouse and rat samples through western blotting (WB) and immunoprecipitation (IP) applications. CYP2B1/2B2 is a crucial member of the cytochrome P450 (CYP2) superfamily, which plays a significant role in the metabolism of various endogenous and exogenous compounds, including therapeutic agents and fatty acids. These enzymes are primarily located in the liver, where they facilitate the oxidation of substances, thereby influencing drug metabolism and detoxification processes. CYP2B1 and CYP2B2 convert prodrugs, such as ifosfamide, into their active cytotoxic forms, which is vital for effective cancer treatment. Additionally, CYP2B2 mediates the induction of these enzymes by phenobarbital, highlighting the regulatory mechanisms that can affect drug metabolism. Anti-CYP2B1/2B2 antibody (h7) serves as an essential tool for researchers studying the pharmacokinetics of drugs and the metabolic pathways involving these critical enzymes, providing insights into their function and interactions within the hepatic environment.

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CYP2B1/2B2 Antibody (h7) References:

1. Microencapsulated, CYP2B1-transfected cells activating ifosfamide at the site of the tumor: the magic bullets of the 21st century.  |  Löhr, M., et al. 2002. Cancer Chemother Pharmacol. 49 Suppl 1: S21-4. PMID: 12042985
2. Lack of evidence for induction of CYP2B1, CYP3A23, and CYP1A2 gene expression by Panax ginseng and Panax quinquefolius extracts in adult rats and primary cultures of rat hepatocytes.  |  Yu, CT., et al. 2005. Drug Metab Dispos. 33: 19-22. PMID: 15466162
3. The CYP2B2 5' flank contains a complex glucocorticoid response unit.  |  Audet-Walsh, E., et al. 2008. Biochem Pharmacol. 76: 1298-306. PMID: 18782568
4. Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats.  |  Kobayashi, M., et al. 2014. Biosci Biotechnol Biochem. 78: 1060-6. PMID: 25036135
5. Effects of Nonalcoholic Fatty Liver Disease on Hepatic CYP2B1 and in Vivo Bupropion Disposition in Rats Fed a High-Fat or Methionine/Choline-Deficient Diet.  |  Cho, SJ., et al. 2016. J Agric Food Chem. 64: 5598-606. PMID: 27321734
6. Bromuconazole-induced hepatotoxicity is accompanied by upregulation of PXR/CYP3A1 and downregulation of CAR/CYP2B1 gene expression.  |  Abdelhadya, DH., et al. 2017. Toxicol Mech Methods. 27: 544-550. PMID: 28532222
7. Localization of a phenobarbital-responsive element (PBRE) in the 5'-flanking region of the rat CYP2B2 gene.  |  Trottier, E., et al. 1995. Gene. 158: 263-8. PMID: 7607552
8. New proteins in the rat CYP2B subfamily: presence in liver microsomes of the constitutive CYP2B3 protein and the phenobarbital-inducible protein product of alternatively spliced CYP2B2 mRNA.  |  Desrochers, M., et al. 1996. Biochem Pharmacol. 52: 1311-9. PMID: 8937440
9. Effect of ovariectomy and androgen on phenobarbital induction of hepatic CYP2B1 and CYP2B2 in Sprague-Dawley rats.  |  Chang, TK., et al. 1997. Drug Metab Dispos. 25: 994-1000. PMID: 9280408
10. The CYP2B2 phenobarbital response unit contains an accessory factor element and a putative glucocorticoid response element essential for conferring maximal phenobarbital responsiveness.  |  Stoltz, C., et al. 1998. J Biol Chem. 273: 8528-36. PMID: 9525968

The preferred CYP2B1/2B2 antibody is CYP2B1/2B2 Antibody (9.14): sc-73546.