CYP27B1 Antibody (G-5): sc-515903

CYP27B1 Antibody (G-5) is a mouse monoclonal IgM antibody that detects CYP27B1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CYP27B1 (G-5) antibody is available as the non-conjugated form. CYP27B1, a member of the cytochrome P450 family, plays a crucial role in vitamin D metabolism, specifically catalyzing the conversion of 25-hydroxyvitamin D3 (25(OH)D) to its active form, 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D). This enzymatic activity maintains calcium homeostasis, promotes normal bone growth, and facilitates tissue differentiation. Proper functioning of CYP27B1 remains essential, as deficiencies or mutations in the gene encoding CYP27B1 can lead to vitamin D-dependent rickets type 1 (VDDR-1), a serious condition characterized by rickets, hypocalcemia, and muscle weakness. Localization of CYP27B1 in kidney cell mitochondria underscores CYP27B1′s importance in renal physiology and systemic calcium regulation. Using anti-CYP27B1 antibody (G-5) in various applications helps researchers gain insights into CYP27B1′s role in vitamin D metabolism and related disorders.

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CYP27B1 Antibody (G-5) References:

1. Targeted inactivation of the 25-hydroxyvitamin D(3)-1(alpha)-hydroxylase gene (CYP27B1) creates an animal model of pseudovitamin D-deficiency rickets.  |  Dardenne, O., et al. 2001. Endocrinology. 142: 3135-41. PMID: 11416036
2. Structure-function analysis of CYP27B1 and CYP27A1. Studies on mutants from patients with vitamin D-dependent rickets type I (VDDR-I) and cerebrotendinous xanthomatosis (CTX).  |  Sawada, N., et al. 2001. Eur J Biochem. 268: 6607-15. PMID: 11737215
3. Correction of the abnormal mineral ion homeostasis with a high-calcium, high-phosphorus, high-lactose diet rescues the PDDR phenotype of mice deficient for the 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1).  |  Dardenne, O., et al. 2003. Bone. 32: 332-40. PMID: 12689675
4. Modulation of CYP27B1 and CYP24 mRNA expression in bone is independent of circulating 1,25(OH)2D3 levels.  |  Anderson, PH., et al. 2005. Bone. 36: 654-62. PMID: 15781002
5. Identification of growth factor independent-1 (GFI1) as a repressor of 25-hydroxyvitamin D 1-alpha hydroxylase (CYP27B1) gene expression in human prostate cancer cells.  |  Dwivedi, PP., et al. 2005. Endocr Relat Cancer. 12: 351-65. PMID: 15947108
6. A haplotype of the CYP27B1 promoter is associated with autoimmune Addison's disease but not with Graves' disease in a UK population.  |  Jennings, CE., et al. 2005. J Mol Endocrinol. 34: 859-63. PMID: 15956353
7. Identification of the amino acid residue of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D-dependent rickets type 1.  |  Yamamoto, K., et al. 2005. J Biol Chem. 280: 30511-6. PMID: 15972816
8. Vitamin D(3) metabolism in human glioblastoma multiforme: functionality of CYP27B1 splice variants, metabolism of calcidiol, and effect of calcitriol.  |  Diesel, B., et al. 2005. Clin Cancer Res. 11: 5370-80. PMID: 16061850
9. CYP27B1 gene polymorphism is associated with Graves' disease in a Polish population study.  |  Kurylowicz, A. and Badenhoop, K. 2005. Thyroid. 15: 1107-8. PMID: 16187925
10. Vitamin D receptor (VDR) and metabolizing enzymes CYP27B1 and CYP24A1 in breast cancer.  |  Voutsadakis, IA. 2020. Mol Biol Rep. 47: 9821-9830. PMID: 33259013
11. 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification.  |  Yimamu, Y., et al. 2022. J Clin Biochem Nutr. 71: 103-111. PMID: 36213783
12. Polymorphisms in VDR, CYP27B1, CYP2R1, GC and CYP24A1 Genes as Biomarkers of Survival in Non-Small Cell Lung Cancer: A Systematic Review.  |  Pineda-Lancheros, LE., et al. 2023. Nutrients. 15: PMID: 36986255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CYP27B1 Antibody (G-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CYP27B1 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CYP27B1 Antibody (G-5) Specifications

Alternate Product Name: Cytochrome P450 27B1

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 468-486

Epitope Target: CYP27B1

Epitope Species: human

Product Reactivity/Detection: CYP27B1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CYP27B1 Antibody (G-5): N/A