cyclin T1 Antibody (E-3): sc-271348

cyclin T1 Antibody (E-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects cyclin T1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). cyclin T1 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyclin T1 plays a crucial role in the regulation of transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for the phosphorylation of the C-terminal domain of RNA polymerase II. This phosphorylation is vital for the transition from transcription initiation to elongation, thereby influencing gene expression. Notably, cyclin T1 interacts with the HIV-1 Tat protein, enhancing its binding affinity to the viral TAR RNA, which is critical for the recruitment of the cyclin T1/Cdk9 complex to RNA polymerase II. The human positive transcription elongation factor b (P-TEFb) is composed of Cdk9 and either cyclin T1 or cyclin T2, with cyclin T1 being specifically required for the Tat-mediated transcriptional activation of HIV-1. The unique interaction of cyclin T1 with Tat, as opposed to cyclin T2, underscores its importance in viral transcription and highlights its potential as a target for therapeutic intervention in HIV-related diseases.

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cyclin T1 Antibody (E-3) References:

1. Interactions between Tat and TAR and human immunodeficiency virus replication are facilitated by human cyclin T1 but not cyclins T2a or T2b.  |  Wimmer, J., et al. 1999. Virology. 255: 182-9. PMID: 10049833
2. The CDK9-associated cyclins T1 and T2 exert opposite effects on HIV-1 Tat activity.  |  Napolitano, G., et al. 1999. AIDS. 13: 1453-9. PMID: 10465067
3. Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells.  |  Sung, TL. and Rice, AP. 2006. Retrovirology. 3: 66. PMID: 17014716
4. Arginine methylation of the human immunodeficiency virus type 1 Tat protein by PRMT6 negatively affects Tat Interactions with both cyclin T1 and the Tat transactivation region.  |  Xie, B., et al. 2007. J Virol. 81: 4226-34. PMID: 17267505
5. Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription.  |  Wang, Q., et al. 2007. J Mol Biol. 367: 630-46. PMID: 17289077
6. Inhibition of the CDK9-cyclin T1 protein-protein interaction as a new approach against triple-negative breast cancer.  |  Cheng, SS., et al. 2022. Acta Pharm Sin B. 12: 1390-1405. PMID: 35530158
7. Discovery of Small-Molecule Degraders of the CDK9-Cyclin T1 Complex for Targeting Transcriptional Addiction in Prostate Cancer.  |  Li, J., et al. 2022. J Med Chem. 65: 11034-11057. PMID: 35925880
8. Discovery of HyT-Based Degraders of CDK9-Cyclin T1 Complex.  |  Lin, R., et al. 2023. Chem Biodivers. 20: e202300769. PMID: 37349855
9. Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor.  |  Herrmann, CH. and Rice, AP. 1995. J Virol. 69: 1612-20. PMID: 7853496
10. TAK, an HIV Tat-associated kinase, is a member of the cyclin-dependent family of protein kinases and is induced by activation of peripheral blood lymphocytes and differentiation of promonocytic cell lines.  |  Yang, X., et al. 1997. Proc Natl Acad Sci U S A. 94: 12331-6. PMID: 9356449
11. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA.  |  Wei, P., et al. 1998. Cell. 92: 451-62. PMID: 9491887
12. Identification of multiple cyclin subunits of human P-TEFb.  |  Peng, J., et al. 1998. Genes Dev. 12: 755-62. PMID: 9499409

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin T1 Antibody (E-3) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin T1 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin T1 Antibody (E-3) Specifications

Alternate Product Name: cyclin T1

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 671-708

Epitope Target: cyclin T1

Epitope Species: human

Product Reactivity/Detection: cyclin T1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for cyclin T1 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent