cyclin K Antibody (G-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 107-286 mapping within an internal region of cyclin K of human origin
cyclin K Antibody (G-11) is recommended for detection of cyclin K of mouse, rat and human origin by WB, IP, IF and ELISA
cyclin K Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin K (G-11): sc-376371.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Positive transcription elongation factor b (P-TEFb) complexes are crucial for allowing the elongation of RNA by RNA polymerase II (RNAPII). These complexes are able to phosphorylate the carboxyl-terminal domain of the largest RNAPII subunit. P-TEFb complexes are made up of a catalytic subunit, cyclin dependent kinase 9 (Cdk9), and one of the regulatory cyclins, CycT1, CycT2a, CycT2b or cyclin K. Specifically, cyclin K forms an active P-TEFb complex with Cdk9. This complex promotes transcription by phosphorylating the carboxyl-terminal domain of RNAPII which allows the elongation of transcription to proceed. Cyclin K is ubiquitously expressed in adult mouse and human tissues, with highest levels expressed in the developing germ cells of adult testis and ovaries. Cyclin K is also present in HepG2 cells. The cyclin K gene encodes a 357 amino acid protein and maps to human chromosome 14q32.2.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Are there any issues with using sc-376371: cyclin K (G-11) mouse monoclonal primary antibody on mouse samples?
Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376371 conjugates.
Rated 3 out of
an OK antibody for WBThis antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, the intensity is somewhat weak.
Date published: 2017-09-18
Rated 5 out of
Works as expected in WBThis cyclin K monoclonal produced a clean strong WB band in SW480 and c4 (C) whole cell lysates.
Date published: 2017-02-01
Rated 5 out of
Great product!A single band at the expected molecular weight was detected in Hela cell total lysate.
Date published: 2016-12-17
Rated 5 out of
good productWB is clean and good, highly recommended! Will keep use it.
Date published: 2016-11-21
Rated 5 out of
Good specificity and signal strength in HeLaGood specificity and signal strength in HeLa, Hep G2 and NTERA-2 cl.D1 whole cell lysates. -SCBT QC
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