cyclin K Antibody (G-11)recommended for detection of cyclin K of mouse, rat and human origin by WB, IP, IF and ELISA

cyclin K Antibody (G-11): sc-376371

cyclin K Antibody (G-11) is rated 4.6 out of 5 by 5.
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Datasheets

    • cyclin K Antibody (G-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 107-286 mapping within an internal region of cyclin K of human origin
    • cyclin K Antibody (G-11) is recommended for detection of cyclin K of mouse, rat and human origin by WB, IP, IF and ELISA
    • cyclin K Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin K (G-11): sc-376371.
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Are there any issues with using sc-376371: cyclin K (G-11) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376371 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from an OK antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, the intensity is somewhat weak.
Date published: 2017-09-18
Rated 5 out of 5 by from Works as expected in WB This cyclin K monoclonal produced a clean strong WB band in SW480 and c4 (C) whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from Great product! A single band at the expected molecular weight was detected in Hela cell total lysate.
Date published: 2016-12-17
Rated 5 out of 5 by from good product WB is clean and good, highly recommended! Will keep use it.
Date published: 2016-11-21
Rated 5 out of 5 by from Good specificity and signal strength in HeLa Good specificity and signal strength in HeLa, Hep G2 and NTERA-2 cl.D1 whole cell lysates. -SCBT QC
Date published: 2015-02-12
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