cyclin K Antibody (G-11): sc-376371
- cyclin K Antibody (G-11) is a mouse monoclonal IgG1 κ cyclin K antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 107-286 mapping within an internal region of cyclin K of human origin
- cyclin K Antibody (G-11) is recommended for detection of cyclin K of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyclin K Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for cyclin K Antibody (G-11) for WB applications. This reagent is now offered in a bundle with cyclin K Antibody (G-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Cyclin K Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin K protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cyclin K antibody (G-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cyclin K plays a pivotal role in forming positive transcription elongation factor b (P-TEFb) complexes, which are essential for RNA elongation by RNA polymerase II (RNAPII). These complexes, composed of catalytic subunit cyclin-dependent kinase 9 (Cdk9) and regulatory cyclins such as CycT1, CycT2a, CycT2b, or cyclin K, facilitate phosphorylation of the carboxyl-terminal domain of the largest RNAPII subunit, promoting transcriptional elongation. Cyclin K directly influences gene expression and cellular function through this process. Cyclin K shows ubiquitous expression across various adult mouse and human tissues, with particularly high levels in developing germ cells of adult testis and ovaries, as well as in HepG2 cells. Cyclin K gene encodes a 357 amino acid protein and resides on human chromosome 14q32.2, highlighting significance in both normal physiology and potential disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin K Antibody (G-11) References:
- Cyclin K functions as a CDK9 regulatory subunit and participates in RNA polymerase II transcription. | Fu, TJ., et al. 1999. J Biol Chem. 274: 34527-30. PMID: 10574912
- P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. | Lin, X., et al. 2002. J Biol Chem. 277: 16873-8. PMID: 11884399
- Cyclin K as a direct transcriptional target of the p53 tumor suppressor. | Mori, T., et al. 2002. Neoplasia. 4: 268-74. PMID: 11988847
- Kaposi sarcoma-associated viral cyclin K overrides cell growth inhibition mediated by oncostatin M through STAT3 inhibition. | Lundquist, A., et al. 2003. Blood. 101: 4070-7. PMID: 12531804
- Cyclin-dependent kinase 9-cyclin K functions in the replication stress response. | Yu, DS., et al. 2010. EMBO Rep. 11: 876-82. PMID: 20930849
- Cyclin K-containing kinase complexes maintain self-renewal in murine embryonic stem cells. | Dai, Q., et al. 2012. J Biol Chem. 287: 25344-52. PMID: 22547058
- A distinct expression pattern of cyclin K in mammalian testes suggests a functional role in spermatogenesis. | Xiang, X., et al. 2014. PLoS One. 9: e101539. PMID: 25004108
- Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer. | Schecher, S., et al. 2017. Int J Cancer. 141: 1643-1653. PMID: 28670704
- HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity. | Chai, Q., et al. 2021. Cell Rep. 36: 109514. PMID: 34380030
- Structural Mass Spectrometry Probes the Inhibitor-Induced Allosteric Activation of CDK12/CDK13-Cyclin K Dissociation. | Bai, Y., et al. 2023. J Am Chem Soc. 145: 11477-11481. PMID: 37207290
- Inhibition of the Cyclin K-CDK12 complex induces DNA damage and increases the effect of androgen deprivation therapy in prostate cancer. | Frei, K., et al. 2024. Int J Cancer. 154: 1082-1096. PMID: 37916780
- Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity. | Edwards, MC., et al. 1998. Mol Cell Biol. 18: 4291-300. PMID: 9632813
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin K Antibody (G-11) | sc-376371 | 200 µg/ml | $322.00 | |||
cyclin K Antibody (G-11): m-IgGκ BP-HRP Bundle | sc-552039 | 200 µg Ab; 40 µg BP | $361.00 | |||
cyclin K Antibody (G-11) AC | sc-376371 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin K Antibody (G-11) HRP | sc-376371 HRP | 200 µg/ml | $322.00 | |||
cyclin K Antibody (G-11) FITC | sc-376371 FITC | 200 µg/ml | $336.00 | |||
cyclin K Antibody (G-11) PE | sc-376371 PE | 200 µg/ml | $349.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 488 | sc-376371 AF488 | 200 µg/ml | $364.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 546 | sc-376371 AF546 | 200 µg/ml | $364.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 594 | sc-376371 AF594 | 200 µg/ml | $364.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 647 | sc-376371 AF647 | 200 µg/ml | $364.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 680 | sc-376371 AF680 | 200 µg/ml | $364.00 | |||
cyclin K Antibody (G-11) Alexa Fluor® 790 | sc-376371 AF790 | 200 µg/ml | $364.00 |