cyclin D2 Antibody (DCS-5): sc-53637

Cyclin D2 Antibody (DCS-5) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect cyclin D2, a pivotal protein involved in regulating the cell cycle. The cyclin D2 protein is characterized by its conserved cyclin box domain, which is essential for binding to cyclin-dependent kinases (Cdk4 and Cdk6), forming active complexes that phosphorylate the retinoblastoma (Rb) protein. This phosphorylation is crucial for the release of E2F transcription factors, facilitating the transition from the G1 phase to the S phase of the cell cycle, thereby promoting DNA synthesis and cell proliferation. The structural integrity of cyclin D2 is vital for its interaction with CDKs and its role in cell cycle progression, making cyclin D2 a key factor in normal cellular growth and a significant contributor to oncogenesis when dysregulated. Anti-cyclin D2 antibody (DCS-5) is raised against the full-length human cyclin D2 protein and demonstrates robust reactivity across mouse, rat, and human species in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, cyclin D2 (DCS-5) antibody provides versatility for diverse research applications. By enabling precise detection and analysis of cyclin D2′s structural and functional roles within the cell, cyclin D2 monoclonal antibody (DCS-5) supports advanced studies in cell cycle regulation and the molecular mechanisms underlying cancer development, making cyclin D2 (DCS-5) antibody an invaluable tool for researchers in the field of oncology and cell biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin D2 Antibody (DCS-5) References:

1. Expression of cyclin D2 and D3 in lymphoid lesions.  |  Teramoto, N., et al. 1999. Int J Cancer. 81: 543-50. PMID: 10225442
2. Up-regulation of cyclin-dependent kinase 4/cyclin D2 expression but down-regulation of cyclin-dependent kinase 2/cyclin E in testicular germ cell tumors.  |  Schmidt, BA., et al. 2001. Cancer Res. 61: 4214-21. PMID: 11358847
3. BCR signals target p27(Kip1) and cyclin D2 via the PI3-K signalling pathway to mediate cell cycle arrest and apoptosis of WEHI 231 B cells.  |  Banerji, L., et al. 2001. Oncogene. 20: 7352-67. PMID: 11704865
4. Characterization of cyclin D2 expression in human endometrium.  |  Choi, D., et al. 2002. J Soc Gynecol Investig. 9: 41-6. PMID: 11839508
5. Absence of cyclin D2 expression is associated with promoter hypermethylation in gastric cancer.  |  Yu, J., et al. 2003. Br J Cancer. 88: 1560-5. PMID: 12771922
6. Inactivation of cyclin D2 gene in prostate cancers by aberrant promoter methylation.  |  Padar, A., et al. 2003. Clin Cancer Res. 9: 4730-4. PMID: 14581343
7. Promoter methylation of cyclin D2 gene in gastric carcinoma.  |  Oshimo, Y., et al. 2003. Int J Oncol. 23: 1663-70. PMID: 14612939
8. Androgen regulation of stage-dependent cyclin D2 expression in Sertoli cells suggests a role in modulating androgen action on spermatogenesis.  |  Tan, KA., et al. 2005. Biol Reprod. 72: 1151-60. PMID: 15659706
9. Aberrant expression of cyclin D2 is an early event in human male germ cell tumorigenesis.  |  Houldsworth, J., et al. 1997. Cell Growth Differ. 8: 293-9. PMID: 9056671
10. Proliferation-independent induction of macrophage cyclin D2, and repression of cyclin D1, by lipopolysaccharide.  |  Vadiveloo, PK., et al. 1998. J Biol Chem. 273: 23104-9. PMID: 9722538

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin D2 Antibody (DCS-5) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin D2 Antibody (DCS-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin D2 Antibody (DCS-5) Specifications

Alternate Product Name: cyclin D2

Product Clone Name: DCS-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: cyclin D2

Epitope Species: human

Product Reactivity/Detection: cyclin D2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for cyclin D2 Antibody (DCS-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred cyclin D1 antibody is cyclin D1 Antibody (DCS-6): sc-20044.