Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cyclin D1 CRISPR/Cas9 KO Plasmid (h): sc-400047
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin D1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cyclin D1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin D1 Antibody (A-12): sc-8396
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin D1 CRISPR/Cas9 KO Plasmid (h) | sc-400047 | 20 µg | $397.00 |
Overview
CCND1 encodes cyclin D1, a core regulator of the G1/S cell-cycle transition that activates CDK4/6 to phosphorylate RB family proteins and promote E2F-dependent transcription. Cyclin D1 integrates mitogenic and nutrient signals downstream of pathways such as RTK–RAS–MAPK and PI3K–AKT, coordinating proliferation with growth control and checkpoint integrity. Dysregulated CCND1 expression or copy number gain is frequently associated with aberrant cell-cycle entry and genomic instability, making it a common molecular feature studied in tumorigenesis and oncogenic signaling networks.
cyclin D1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCND1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCND1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCND1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cyclin D1 protein expression.
This CRISPR knockout system enables efficient generation of CCND1-deficient cell models for investigation of cyclin D1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCND1 exon(s) critical for cyclin D1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCND1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cyclin D1 CRISPR/Cas9 KO Plasmid (h) and cyclin D1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCND1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cyclin D1 HDR Plasmid (h) and cyclin D1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCND1 homology arms to support homology-directed repair at defined CCND1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.