cyclin B3 Antibody (E-8): sc-515887
- cyclin B3 Antibody (E-8) is a mouse monoclonal IgG1 κ cyclin B3 antibody provided at 200 µg/ml
- raised against amino acids 1-60 mapping at the N-terminus of cyclin B3 of human origin
- cyclin B3 Antibody (E-8) is recommended for detection of cyclin B3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyclin B3 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin B3 Antibody (E-8) for WB applications. These reagents are now offered in bundles with cyclin B3 Antibody (E-8) (see ordering information below).
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cyclin B3 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin B3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). cyclin B3 Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyclin B3, also known as CCNB3 or CYCB3, is a 1,395 amino acid nuclear protein that plays a crucial role in regulating meiotic cell cycle, particularly during early meiotic prophase I. cyclin B3 is predominantly expressed in testis, with lower levels found in other tissues, highlighting specific function in gametogenesis. The cyclin family, which includes cyclins A, B, C, D, E, F, G, H, I, K, L, and T, along with related proteins, such as Dbf4, are essential for forming active protein kinase complexes that control cell cycle progression. Expression and stability of cyclins, including cyclin B3, fluctuate throughout cell cycle, which is vital for precise timing of kinase activation and subsequent cell cycle transitions. cyclin B3 exists in three isoforms due to alternative splicing, which may contribute to functional diversity and regulatory mechanisms in meiosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin B3 Antibody (E-8) References:
- Molecular cloning, gene localization, and structure of human cyclin B3. | Lozano, JC., et al. 2002. Biochem Biophys Res Commun. 291: 406-13. PMID: 11846420
- Characterization and expression of mammalian cyclin b3, a prepachytene meiotic cyclin. | Nguyen, TB., et al. 2002. J Biol Chem. 277: 41960-9. PMID: 12185076
- The cloning of cyclin B3 and its gene expression during hormonally induced spermatogenesis in the teleost, Anguilla japonica. | Kajiura-Kobayashi, H., et al. 2004. Biochem Biophys Res Commun. 323: 288-92. PMID: 15351735
- Human cyclin B3. mRNA expression during the cell cycle and identification of three novel nonclassical nuclear localization signals. | Tschöp, K., et al. 2006. FEBS J. 273: 1681-95. PMID: 16623705
- Misexpression of cyclin B3 leads to aberrant spermatogenesis. | Refik-Rogers, J., et al. 2006. Cell Cycle. 5: 1966-73. PMID: 16929180
- Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A- and B-type cyclins. | Gallant, P. and Nigg, EA. 1994. EMBO J. 13: 595-605. PMID: 8313904
- Dynamics of cell cycle regulators: artifact-free analysis by recultivation of cells synchronized by centrifugal elutriation. | Mikulits, W., et al. 1997. DNA Cell Biol. 16: 849-59. PMID: 9260928
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin B3 Antibody (E-8) | sc-515887 | 200 µg/ml | $322.00 | |||
cyclin B3 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-531550 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin B3 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-525268 | 200 µg Ab, 40 µg BP | $361.00 | |||
cyclin B3 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544616 | 200 µg Ab; 20 µg BP | $361.00 | |||
cyclin B3 Antibody (E-8) AC | sc-515887 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin B3 Antibody (E-8) HRP | sc-515887 HRP | 200 µg/ml | $322.00 | |||
cyclin B3 Antibody (E-8) FITC | sc-515887 FITC | 200 µg/ml | $336.00 | |||
cyclin B3 Antibody (E-8) PE | sc-515887 PE | 200 µg/ml | $349.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 488 | sc-515887 AF488 | 200 µg/ml | $364.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 546 | sc-515887 AF546 | 200 µg/ml | $364.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 594 | sc-515887 AF594 | 200 µg/ml | $364.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 647 | sc-515887 AF647 | 200 µg/ml | $364.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 680 | sc-515887 AF680 | 200 µg/ml | $364.00 | |||
cyclin B3 Antibody (E-8) Alexa Fluor® 790 | sc-515887 AF790 | 200 µg/ml | $364.00 |