cyclin B2 Antibody (A-2): sc-28303

cyclin B2 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin B2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-cyclin B2 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. cyclin B2 plays a crucial role in regulating cell cycle, particularly during transition from G2 phase to mitosis. cyclin B2 functions by forming a complex with cyclin-dependent kinase Cdc2, which is essential for activating maturation-promoting factor (MPF). This activation is vital for proper progression of mitosis, triggering phosphorylation of various substrates that lead to chromatin condensation, nuclear envelope breakdown, and spindle formation. Precise regulation of cyclin B2 and Cdc2 interaction is critical, as dysregulation can lead to uncontrolled cell division and is often implicated in cancer. cyclin B2 monoclonal antibody (A-2) serves as a valuable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying mitotic regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

cyclin B2 Antibody (A-2) References:

1. Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation.  |  Gautier, J. and Maller, JL. 1991. EMBO J. 10: 177-82. PMID: 1824935
2. Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
3. Cyclin is a component of maturation-promoting factor from Xenopus.  |  Gautier, J., et al. 1990. Cell. 60: 487-94. PMID: 1967981
4. Control of M-phase by maturation-promoting factor.  |  Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
5. Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps.  |  Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
6. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
7. Dominoes and clocks: the union of two views of the cell cycle.  |  Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
8. The cyclin B2/CDK1 complex inhibits separase activity in mouse oocyte meiosis I.  |  Li, J., et al. 2019. Development. 146: PMID: 31704793
9. The Cyclin B2/CDK1 Complex Conservatively Inhibits Separase Activity in Oocyte Meiosis II.  |  Li, J., et al. 2021. Front Cell Dev Biol. 9: 648053. PMID: 33777955
10. Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.  |  Liu, S., et al. 2022. EMBO Rep. 23: e54171. PMID: 35384228
11. Extracellular vesicle-derived CircWhsc1 promotes cardiomyocyte proliferation and heart repair by activating TRIM59/STAT3/Cyclin B2 pathway.  |  Wei, G., et al. 2022. J Adv Res.. PMID: 36587763
12. Isolation of the murine cyclin B2 cDNA and characterization of the lineage and temporal specificity of expression of the B1 and B2 cyclins during oogenesis, spermatogenesis and early embryogenesis.  |  Chapman, DL. and Wolgemuth, DJ. 1993. Development. 118: 229-40. PMID: 8375336

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin B2 Antibody (A-2) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin B2 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin B2 Antibody (A-2) Specifications

Alternate Product Name: cyclin B2

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-105

Epitope Target: cyclin B2

Epitope Species: human

Product Reactivity/Detection: cyclin B2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for cyclin B2 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin