CCNB1/cyclin B1 Antibody (D-11): sc-7393

Cyclin B1 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-433, representing the full-length human cyclin B1 protein, also known as CCNB1. cyclin B1 monoclonal antibody (D-11) reacts with mouse, rat, and human species and is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cyclin B1 is a crucial regulatory protein involved in controlling the G2/M transition during the cell cycle by forming a complex with CDK1 (also known as CDC2) to create the maturation-promoting factor (MPF). Cyclin B1 is predominantly localized in the cytoplasm during interphase but accumulates in the nucleus at the onset of mitosis, which is essential for initiating mitotic events. Nuclear translocation of cyclin B1 activates CDK1, leading to the phosphorylation of target proteins that drive cell division. Dysregulation of cyclin B1 expression or localization can result in uncontrolled cell proliferation and is associated with various cancers, highlighting cyclin B1′s significance in cell cycle regulation and oncogenesis. Cyclin B1 monoclonal antibody (D-11) enables researchers to study expression patterns and subcellular localization of cyclin B1, providing insights into normal cellular processes and disease states. Cyclin B1 (D-11) antibody serves as a valuable tool for advancing research in cell biology and developing potential therapeutic interventions targeting cell cycle dysregulation.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CCNB1/cyclin B1 Antibody (D-11) has 116 citations in a variety of scientific publications.

Primary antibodies like Anti-CCNB1/cyclin B1 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CCNB1/cyclin B1 Antibody (D-11) Specifications

Alternate Product Name: cyclin B1

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-433

Epitope Target: cyclin B1

Epitope Species: human

Product Reactivity/Detection: cyclin B1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 116 citations in various scientific publications

Available Conjugates/Forms for CCNB1/cyclin B1 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CCNB1/cyclin B1 antibody is CCNB1/cyclin B1 Antibody (GNS1): sc-245.