Anti-cyclin B1 Antibody (D-11) recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-cyclin B1 Antibody (D-11): sc-7393

Anti-cyclin B1 Antibody (D-11) is rated 4.5 out of 5 by 11.
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Datasheets

    • cyclin B1 Antibody (D-11) is a mouse monoclonal IgG1 κ, cited in 102 publications, provided at 200 µg/ml
    • raised against amino acids 1-433 representing full length cyclin B1 of human origin
    • Anti-cyclin B1 Antibody (D-11) is recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-cyclin B1 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin B1 (D-11): sc-7393.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin B1 Antibody (D-11) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin B1 Antibody (D-11) (see ordering information below).
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请问怎么获得SC-7393的免费小样,我想先试用下,如果质量好,我再购买

Asked by: Dr Wang
感谢您的提问。如何获取免费试用装,请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Can this cyclin B1 (D-11): sc-7393 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer this antibody directly conjugated a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 3 out of 5 by from I don't know Used for Immunoblotting, probed 1:500 in tbs 0.1% tween overnight, it produces a faint signal slightly higher than the expected molecular weight on WB.
Date published: 2021-10-04
Rated 3 out of 5 by from Faint Western Detection in Rat INS-1 Cell Line 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a faint band of ~55 kD. The attached picture is a triplicate set (identical lanes)
Date published: 2019-05-22
Rated 4 out of 5 by from 功能很出色 两个月前申请试用的小样,条带还不错,位置很正确,用着很顺利,如果正装有小包装,那就更好了~~~~~~
Date published: 2017-12-19
Rated 5 out of 5 by from Happy with the result! I did WB in patient and control Fibroblats. It worked very well! I'm very happy with the result.
Date published: 2017-09-29
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Checking for G2/M phase I was very pleased with my result after using the cyclin B1 antibody (1:500 dilution). I can see the cell cycle trend in my cells after thymidine release.
Date published: 2017-08-24
Rated 5 out of 5 by from Well characterized cyclin B1 monoclonal ab With more than 50 citations in the primary literature, this Cyclin B1 clone is well established and liked in a wide variety of applications.
Date published: 2016-12-30
Rated 5 out of 5 by from Great WB data obtained using HeLa and K Great WB data obtained using HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-12
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