cyclin B1 Antibody (0.N.238) recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA

cyclin B1 Antibody (0.N.238): sc-70898

cyclin B1 Antibody (0.N.238) is rated 4.5 out of 5 by 2.
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Datasheets

    • cyclin B1 Antibody (0.N.238) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • recommended for detection of cyclin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and KA
    • See cyclin B1 (GNS1): sc-245 for cyclin B1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for cyclin B1 Antibody (0.N.238) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin B1 Antibody (0.N.238) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Band at expected size with transfected lysate Band at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Published data in IF application with U2OS Published data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
Date published: 2013-01-22
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