Anti-cyclin A Antibody (B-8) recommended for detection of cyclin A and cyclin A1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-cyclin A Antibody (B-8): sc-271682

Anti-cyclin A Antibody (B-8) is rated 4.6 out of 5 by 34.
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Datasheets

    • Anti-cyclin A Antibody (B-8) is a mouse monoclonal IgG2a κ cyclin A antibody, cited in 71 publications, provided at 200 µg/ml
    • raised against amino acids 1-432 representing full length cyclin A of human origin
    • cyclin A Antibody (B-8) is recommended for detection of cyclin A and cyclin A1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-cyclin A Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271682 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin A (B-8): sc-271682.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for cyclin A Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin A Antibody (B-8) (see ordering information below).
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Does it work for IF using Rat or Mouse tissue?

Asked by: Tiago D
Thank you for your question. This antibody has not been tested for use on any other species than human samples, so we only recommend and warranty it for use on human samples. It may work in other species, but we can not warranty it for that use. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-26

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with cyclin A (B-8): sc-271682?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-12
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Rated 5 out of 5 by from WB results 刚才没看货号就评价了,尴尬!我们用的是这个货号,免疫印迹实验结果非常好,1:1000就可以做出来,或许与样品来源相关,强烈推荐啊。
Date published: 2020-11-15
Rated 4 out of 5 by from Works well for western blot and IP I have used this antibody for WB 1:500. It recognises a band at 54 kDa. However it also detects a band around 102 kDa. It also perform well for IP, 2 ug with 500ug lysate for RPE1 cells 2h RT. Looking forward to test if for IF.
Date published: 2020-09-30
Rated 5 out of 5 by from 免疫沈降で使用 Immunoprecipitationで使用。5ul/sampleで用いた。非常に良好に免疫沈降することができた。
Date published: 2020-06-19
Rated 4 out of 5 by from Good for immunofluorescent staining The antibody can give a specific staining profile matches with Cyclin A with 1:500 dilution. Samples were HeLa cells fixed with cold methanol.
Date published: 2019-10-08
Rated 4 out of 5 by from ok on WB Antibody detect a specific band at 50Kda on human cheratinocytes extracts
Date published: 2018-12-12
Rated 5 out of 5 by from good product It is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 5 out of 5 by from Works well in IF Works well in IF in blocking buffer (BSA 3%, Triton 0.1%). I used it at 1/200
Date published: 2018-07-16
Rated 4 out of 5 by from Detects correct 54kDa band Antibody detects strong CyclinA 54kDa band in WB (A549 cell lysate), however, also detects 2 higher bands (ca. 70kDa and 100kDa) and two lower bands (ca 40kDa and 25kDa) used 1:200, o.n. incubation at 4C.
Date published: 2018-01-17
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