Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CXXC5 CRISPR/Cas9 KO Plasmid (h): sc-403248
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXXC5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CXXC5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CXXC5 Antibody (H-6): sc-376348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXXC5 CRISPR/Cas9 KO Plasmid (h) | sc-403248 | 20 µg | $397.00 |
Overview
CXXC5 (CXXC-type zinc finger protein 5) is a nuclear and cytoplasmic regulator that binds CpG-rich DNA through its CXXC domain and interfaces with epigenetic control of transcription. It functions as a context-dependent modulator of Wnt/β-catenin signaling and participates in pathways governing cell fate decisions, proliferation, and differentiation, with reported roles in hematopoietic and neural programs. CXXC5 also links growth factor and stress signaling to transcriptional outputs, influencing chromatin-associated processes and gene expression dynamics. Dysregulated CXXC5 expression has been associated with aberrant Wnt signaling and altered differentiation states observed in multiple disease-relevant contexts, supporting its study in mechanisms of development and oncogenic signaling.
CXXC5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CXXC5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CXXC5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CXXC5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CXXC5 protein expression.
This CRISPR knockout system enables efficient generation of CXXC5-deficient cell models for investigation of CXXC5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CXXC5 exon(s) critical for CXXC5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CXXC5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CXXC5 CRISPR/Cas9 KO Plasmid (h) and CXXC5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CXXC5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CXXC5 HDR Plasmid (h) and CXXC5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CXXC5 homology arms to support homology-directed repair at defined CXXC5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.