CUZD1 CRISPR/Cas9 KO Plasmid (m): sc-421190
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CUZD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CUZD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CUZD1 Antibody (E-10): sc-514578
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CUZD1 CRISPR/Cas9 KO Plasmid (m) | sc-421190 | 20 µg | $397.00 |
Overview
Cuzd1 encodes CUZD1, a CUB and zona pellucida–like domain protein implicated in epithelial cell biology and secretory gland function, with reported roles in protein processing and regulated secretion. In mouse tissues, CUZD1 expression is enriched in glandular epithelia and has been linked to pathways governing cell differentiation, tissue remodeling, and responses to inflammatory cues. Altered CUZD1 regulation has been associated with changes in epithelial homeostasis and proliferative programs, making it relevant for mechanistic studies of barrier tissues and exocrine organ physiology. As a context-dependent modulator of cellular state, CUZD1 provides an entry point for dissecting gene networks that couple secretory function to growth and stress signaling.
CUZD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cuzd1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cuzd1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cuzd1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CUZD1 protein expression.
This CRISPR knockout system enables efficient generation of Cuzd1-deficient cell models for investigation of CUZD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cuzd1 exon(s) critical for CUZD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cuzd1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CUZD1 CRISPR/Cas9 KO Plasmid (m) and CUZD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cuzd1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CUZD1 HDR Plasmid (m) and CUZD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cuzd1 homology arms to support homology-directed repair at defined Cuzd1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.