cubilin Antibody (H-3): sc-518059

Cubilin Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects cubilin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cubilin antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cubilin serves as an essential endocytic receptor, playing a critical role in the absorption of vital nutrients such as vitamin B12 and apolipoprotein A1. Cubilin′s unique structure, which includes an amino terminal segment followed by eight epidermal growth factor-like repeats and a series of 27 CUB domains, allows effective binding to ligands and facilitates their internalization. The gene encoding cubilin is located on chromosome 10 and is predominantly expressed in the intestine, kidney, and yolk sac, with significant presence in intestinal membranes. Cubilin interacts with megalin, another receptor crucial for the internalization of cubilin-bound ligands, highlighting cubilin′s importance in nutrient uptake and cellular homeostasis. Mutations in the cubilin gene can lead to megaloblastic anemia 1 (MGA1), an autosomal recessive disorder characterized by vitamin B12 malabsorption, underscoring cubilin′s vital role in human health.

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cubilin Antibody (H-3) References:

1. Mutations in CUBN, encoding the intrinsic factor-vitamin B12 receptor, cubilin, cause hereditary megaloblastic anaemia 1.  |  Aminoff, M., et al. 1999. Nat Genet. 21: 309-13. PMID: 10080186
2. Cubilin P1297L mutation associated with hereditary megaloblastic anemia 1 causes impaired recognition of intrinsic factor-vitamin B(12) by cubilin.  |  Kristiansen, M., et al. 2000. Blood. 96: 405-9. PMID: 10887099
3. Cubilin, a multifunctional epithelial receptor: an overview.  |  Kozyraki, R. 2001. J Mol Med (Berl). 79: 161-7. PMID: 11409706
4. Cubilin and megalin expression and their interaction in the rat intestine: effect of thyroidectomy.  |  Yammani, RR., et al. 2001. Am J Physiol Endocrinol Metab. 281: E900-7. PMID: 11595644
5. Megalin-dependent cubilin-mediated endocytosis is a major pathway for the apical uptake of transferrin in polarized epithelia.  |  Kozyraki, R., et al. 2001. Proc Natl Acad Sci U S A. 98: 12491-6. PMID: 11606717
6. Cubilin dysfunction causes abnormal metabolism of the steroid hormone 25(OH) vitamin D(3).  |  Nykjaer, A., et al. 2001. Proc Natl Acad Sci U S A. 98: 13895-900. PMID: 11717447
7. Cubilin maintains blood levels of HDL and albumin.  |  Aseem, O., et al. 2014. J Am Soc Nephrol. 25: 1028-36. PMID: 24357674
8. Megalin and cubilin in proximal tubule protein reabsorption: from experimental models to human disease.  |  Nielsen, R., et al. 2016. Kidney Int. 89: 58-67. PMID: 26759048
9. Cubilin, the Intrinsic Factor-Vitamin B12 Receptor in Development and Disease.  |  Kozyraki, R. and Cases, O. 2020. Curr Med Chem. 27: 3123-3150. PMID: 30295181
10. Cubilin, the intrinsic factor-vitamin B12 receptor.  |  Kozyraki, R., et al. 2022. Vitam Horm. 119: 65-119. PMID: 35337634
11. Megalin, cubilin, and Dab2 drive endocytic flux in kidney proximal tubule cells.  |  Rbaibi, Y., et al. 2023. Mol Biol Cell. 34: ar74. PMID: 37126375
12. The human intrinsic factor-vitamin B12 receptor, cubilin: molecular characterization and chromosomal mapping of the gene to 10p within the autosomal recessive megaloblastic anemia (MGA1) region.  |  Kozyraki, R., et al. 1998. Blood. 91: 3593-600. PMID: 9572993

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cubilin Antibody (H-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-cubilin Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cubilin Antibody (H-3) Specifications

Alternate Product Name: cubilin

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 50-73

Epitope Target: cubilin

Epitope Species: human

Product Reactivity/Detection: cubilin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for cubilin Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin