CTRB1/2 Antibody (B-3): sc-398721
- CTRB1/2 Antibody (B-3) is a mouse monoclonal IgG2a κ CTRB1/2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 133-160 within an internal region of CTRB1 of human origin
- CTRB1/2 Antibody (B-3) is recommended for detection of Chymotrypsinogen B1 and Chymotrypsin B1 of mouse, rat and human origin, and Chymotrypsinogen B2 and Chymotrypsin B2 of human origin by WB, IP, IF and ELISA
- Anti-CTRB1/2 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CTRB1/2 Antibody (B-3) for WB applications. These reagents are now offered in bundles with CTRB1/2 Antibody (B-3) (see ordering information below).
CTRB1/2 Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Chymotrypsinogen B1, Chymotrypsin B1, Chymotrypsinogen B2, and Chymotrypsin B2 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CTRB1/2 (B-3) antibody is available in non-conjugated form and conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants. Chymotrypsins, including Chymotrypsin B (CTRB1) and Chymotrypsin B2 (CTRB2), are essential digestive enzymes that perform proteolysis by cleaving peptide bonds at the carboxyl side of aromatic amino acids like tyrosine, tryptophan, leucine, and phenylalanine. This enzymatic activity helps break down proteins into smaller peptides and amino acids, which are crucial for nutrient absorption and metabolic processes. These enzymes are synthesized in the pancreas as inactive precursors, Chymotrypsinogen B1 and Chymotrypsinogen B2, and become activated through proteolytic cleavages that create a three-polypeptide structure stabilized by disulfide bonds. Proper regulation of Chymotrypsin activity is essential, as dysregulation can cause digestive disorders and other health issues, making anti-CTRB1/2 antibody (B-3) valuable for studying these proteins in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CTRB1/2 Antibody (B-3) References:
- [Chymotrypsin]. | Katoh, M. 1999. Nihon Rinsho. 57 Suppl: 372-4. PMID: 10503446
- Ala226 to Gly and Ser189 to Asp mutations convert rat chymotrypsin B to a trypsin-like protease. | Jelinek, B., et al. 2004. Protein Eng Des Sel. 17: 127-31. PMID: 15047908
- Chymotrypsin: molecular and catalytic properties. | Appel, W. 1986. Clin Biochem. 19: 317-22. PMID: 3555886
- Isolation and sequence of a rat chymotrypsin B gene. | Bell, GI., et al. 1984. J Biol Chem. 259: 14265-70. PMID: 6209274
- Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase. | Honey, NK., et al. 1984. Somat Cell Mol Genet. 10: 369-76. PMID: 6589790
- Chromosomal assignments of genes for trypsin, chymotrypsin B, and elastase in mouse. | Honey, NK., et al. 1984. Somat Cell Mol Genet. 10: 377-83. PMID: 6589791
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTRB1/2 Antibody (B-3) | sc-398721 | 200 µg/ml | $322.00 | |||
CTRB1/2 Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-530903 | 200 µg Ab; 10 µg BP | $361.00 | |||
CTRB1/2 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-524457 | 200 µg Ab, 40 µg BP | $361.00 | |||
CTRB1/2 Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-547623 | 200 µg Ab; 10 µg BP | $361.00 | |||
CTRB1/2 Antibody (B-3) AC | sc-398721 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CTRB1/2 Antibody (B-3) HRP | sc-398721 HRP | 200 µg/ml | $322.00 | |||
CTRB1/2 Antibody (B-3) FITC | sc-398721 FITC | 200 µg/ml | $336.00 | |||
CTRB1/2 Antibody (B-3) PE | sc-398721 PE | 200 µg/ml | $349.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 488 | sc-398721 AF488 | 200 µg/ml | $364.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 546 | sc-398721 AF546 | 200 µg/ml | $364.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 594 | sc-398721 AF594 | 200 µg/ml | $364.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 647 | sc-398721 AF647 | 200 µg/ml | $364.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 680 | sc-398721 AF680 | 200 µg/ml | $364.00 | |||
CTRB1/2 Antibody (B-3) Alexa Fluor® 790 | sc-398721 AF790 | 200 µg/ml | $364.00 | |||
CTRB1/2 (B-3) Neutralizing Peptide | sc-398721 P | 100 µg/0.5 ml | $69.00 |