CTBS Antibody (RF-18): sc-100489

CTBS Antibody (RF-18) is a mouse monoclonal IgG1 kappa light chain antibody that detects CTBS protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CTBS antibody (RF-18) is available as a non-conjugated monoclonal isotype antibody. CTBS protein, also known as di-N-acetylchitobiase or chitobiase, is a vital component of the glycosyl hydrolase 18 family and is primarily localized in the lysosome. CTBS protein′s role in the degradation of asparagine-linked glycoproteins is crucial for cellular function, as CTBS facilitates the breakdown of glycoproteins that are delivered to lysosomes through endocytosis or autophagy. This catabolic process is essential for the regular turnover of cellular components and maintenance of glycosylation homeostasis. CTBS acts as a glycosidase, specifically cleaving the reducing end N-acetylglucosamine (GlcNAc) from the core chitobiase unit of oligosaccharides. For CTBS to perform this function, lysosomal glycosylasparaginase (AGA) must first remove asparagine from the Asn-linked glycoprotein, thereby exposing the GlcNAc moiety for CTBS to act upon. Proper functioning of CTBS is integral to cellular degradation pathways, highlighting CTBS′s importance in maintaining cellular health and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CTBS Antibody (RF-18) References:

1. Structure of the human gene for lysosomal di-N-acetylchitobiase.  |  Liu, B., et al. 1999. Glycobiology. 9: 589-93. PMID: 10336991
2. Cloning and expression of the cDNA sequence encoding the lysosomal glycosidase di-N-acetylchitobiase.  |  Fisher, KJ. and Aronson, NN. 1992. J Biol Chem. 267: 19607-16. PMID: 1527079
3. Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5).  |  Fisher, KJ. and Aronson, NN. 1992. Mol Cell Biol. 12: 1585-91. PMID: 1549114
4. Lysosomal metabolism of glycoproteins.  |  Winchester, B. 2005. Glycobiology. 15: 1R-15R. PMID: 15647514
5. Characterization of a human core-specific lysosomal {alpha}1,6-mannosidase involved in N-glycan catabolism.  |  Park, C., et al. 2005. J Biol Chem. 280: 37204-16. PMID: 16115860
6. Optimum substrate size and specific anomer requirements for the reducing-end glycoside hydrolase di-N-acetylchitobiase.  |  Aronson, NN. and Halloran, BA. 2006. Biosci Biotechnol Biochem. 70: 1537-41. PMID: 16794344
7. Lysosomal degradation of Asn-linked glycoproteins.  |  Aronson, NN. and Kuranda, MJ. 1989. FASEB J. 3: 2615-22. PMID: 2531691
8. A di-N-acetylchitobiase activity is involved in the lysosomal catabolism of asparagine-linked glycoproteins in rat liver.  |  Kuranda, MJ. and Aronson, NN. 1986. J Biol Chem. 261: 5803-9. PMID: 3084472
9. Lysosomal chitobiase (CTB) and the G-protein gamma 5 subunit (GNG5) genes co-localize to human chromosome 1p22.  |  Ahmad, W., et al. 1995. Cytogenet Cell Genet. 71: 44-6. PMID: 7606925

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CTBS Antibody (RF-18) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CTBS Antibody (RF-18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CTBS Antibody (RF-18) Specifications

Alternate Product Name: CTBS

Product Clone Name: RF-18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-106

Epitope Target: CTBS

Epitope Species: human

Product Reactivity/Detection: CTBS

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CTBS Antibody (RF-18): N/A