CstF-64 Antibody (E-9): sc-398840

CstF-64 Antibody (E-9) is a mouse monoclonal IgG3 antibody that detects CstF-64 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CstF-64 plays a crucial role in the polyadenylation of mRNA precursors, a vital process for gene expression regulation, as CstF-64 is involved in the endonucleolytic cleavage of polyadenylation substrates. CstF-64 is expressed in all somatic cells and is particularly important in B cell activation, where an increase in CstF-64 concentration facilitates the switch of immunoglobulin M (IgM) heavy chain mRNA from membrane-bound to secreted forms, thereby influencing antibody production. Notably, CstF-64 is encoded by the CSTF2 gene located on the X chromosome, while its larger variant, t CstF-64, is produced from an autosomal gene and is predominantly expressed in meiotic and postmeiotic cells in the testis, highlighting its significance in germ cell development. CstF-64′s ability to regulate mRNA processing underscores its importance in maintaining proper cellular function and response, making anti-CstF-64 antibody (E-9) an essential tool for researchers studying gene expression and B cell differentiation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CstF-64 Antibody (E-9) References:

1. Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein.  |  Pérez Cañadillas, JM. and Varani, G. 2003. EMBO J. 22: 2821-30. PMID: 12773396
2. Protein and RNA dynamics play key roles in determining the specific recognition of GU-rich polyadenylation regulatory elements by human Cstf-64 protein.  |  Deka, P., et al. 2005. J Mol Biol. 347: 719-33. PMID: 15769465
3. Enterovirus 71 3C protease cleaves a novel target CstF-64 and inhibits cellular polyadenylation.  |  Weng, KF., et al. 2009. PLoS Pathog. 5: e1000593. PMID: 19779565
4. The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.  |  Hockert, JA., et al. 2010. J Biol Chem. 285: 695-704. PMID: 19887456
5. Transcriptome-wide analyses of CstF64-RNA interactions in global regulation of mRNA alternative polyadenylation.  |  Yao, C., et al. 2012. Proc Natl Acad Sci U S A. 109: 18773-8. PMID: 23112178
6. Overlapping and distinct functions of CstF64 and CstF64τ in mammalian mRNA 3' processing.  |  Yao, C., et al. 2013. RNA. 19: 1781-90. PMID: 24149845
7. CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding PAPα.  |  Kandala, DT., et al. 2016. Nucleic Acids Res. 44: 811-23. PMID: 26496945
8. PAPERCLIP Identifies MicroRNA Targets and a Role of CstF64/64tau in Promoting Non-canonical poly(A) Site Usage.  |  Hwang, HW., et al. 2016. Cell Rep. 15: 423-35. PMID: 27050522
9. The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.  |  Grozdanov, PN., et al. 2018. Nucleic Acids Res. 46: 12022-12039. PMID: 30257008
10. CstF64-Induced Shortening of the BID 3'UTR Promotes Esophageal Squamous Cell Carcinoma Progression by Disrupting ceRNA Cross-talk with ZFP36L2.  |  Lin, A., et al. 2021. Cancer Res. 81: 5638-5651. PMID: 34607841

The preferred CstF-64 antibody is CstF-64 Antibody (H-1): sc-398862.