CSP CRISPR/Cas9 KO Plasmid (h): sc-403622
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CSP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSP Antibody (H-3): sc-137128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSP CRISPR/Cas9 KO Plasmid (h) | sc-403622 | 20 µg | $397.00 |
Overview
DNAJC5 encodes cysteine string protein (CSP), a palmitoylated J-domain co-chaperone enriched on synaptic vesicles where it coordinates Hsc70-dependent protein quality control at presynaptic terminals. CSP supports efficient synaptic vesicle exocytosis by stabilizing components of the SNARE machinery and other vesicle trafficking proteins, thereby helping maintain neurotransmission under high activity. Through its roles in chaperone-mediated proteostasis, membrane trafficking, and synaptic homeostasis, altered DNAJC5/CSP function is linked to neurodegenerative phenotypes and neuronal vulnerability in disorders characterized by impaired synaptic maintenance.
CSP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJC5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJC5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJC5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CSP protein expression.
This CRISPR knockout system enables efficient generation of DNAJC5-deficient cell models for investigation of CSP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJC5 exon(s) critical for CSP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJC5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CSP CRISPR/Cas9 KO Plasmid (h) and CSP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJC5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CSP HDR Plasmid (h) and CSP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJC5 homology arms to support homology-directed repair at defined DNAJC5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.