CSP Antibody (H-3): sc-137128
- CSP Antibody (H-3) is a mouse monoclonal IgG1 κ CSP antibody provided at 200 µg/ml
- raised against amino acids 71-198 mapping at the C-terminus of CSP of human origin
- CSP Antibody (H-3) is recommended for detection of CSP isoforms 1 and 2 and β-CSP of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine
- Anti-CSP Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CSP Antibody (H-3) for WB applications. These reagents are now offered in bundles with CSP Antibody (H-3) (see ordering information below).
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CSP Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects CSP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CSP antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cysteine string proteins (CSPs) play a crucial role in regulating synaptic transmission, as CSPs are associated with synaptic and secretory vesicles and are involved in calcium-regulated exocytosis. CSP contains a J-domain that interacts with heat shock protein 70 (HSP70) and heat shock cognate protein 70 (HSC70) chaperones, facilitating proper folding of proteins essential for vesicular exocytosis. Additionally, CSPs are characterized by a palmitoylated cysteine-rich string region that aids in membrane targeting, which is vital for their function in synaptic vesicle dynamics. CSP interaction with receptor-coupled trimeric GTP-binding proteins and N-type calcium channels underscores CSP importance in fine-tuning neurotransmission. Notably, two isoforms of CSP have been identified: CSP1, which is predominantly found in granular fractions of insulinoma cells, and CSP2, a truncated variant that is more prevalent in membrane and cytosolic fractions. Differential expression of these isoforms in various mammalian cell lines, including rat brain, highlights CSP′s role in cellular signaling and neurotransmitter release.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CSP Antibody (H-3) References:
- Mutational analysis of cysteine-string protein function in insulin exocytosis. | Zhang, H., et al. 1999. J Cell Sci. 112 (Pt 9): 1345-51. PMID: 10194413
- Cysteine-string protein: the chaperone at the synapse. | Chamberlain, LH. and Burgoyne, RD. 2000. J Neurochem. 74: 1781-9. PMID: 10800920
- Cysteine string protein regulates G protein modulation of N-type calcium channels. | Magga, JM., et al. 2000. Neuron. 28: 195-204. PMID: 11086994
- Cysteine string protein expression in mammary epithelial cells. | Gleave, TL., et al. 2001. Pflugers Arch. 441: 639-49. PMID: 11294245
- Down-regulated expression of exocytotic proteins in pancreatic islets of diabetic GK rats. | Zhang, W., et al. 2002. Biochem Biophys Res Commun. 291: 1038-44. PMID: 11866470
- Cysteine string protein functions directly in regulated exocytosis. | Chamberlain, LH. and Burgoyne, RD. 1998. Mol Biol Cell. 9: 2259-67. PMID: 9693380
- Cysteine string protein (CSP) is an insulin secretory granule-associated protein regulating beta-cell exocytosis. | Brown, H., et al. 1998. EMBO J. 17: 5048-58. PMID: 9724640
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSP Antibody (H-3) | sc-137128 | 200 µg/ml | $316.00 | |||
CSP Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-528864 | 200 µg Ab; 10 µg BP | $354.00 | |||
CSP Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-521432 | 200 µg Ab, 40 µg BP | $354.00 | |||
CSP Antibody (H-3): m-IgG1 BP-HRP Bundle | sc-543176 | 200 µg Ab; 20 µg BP | $354.00 | |||
CSP Antibody (H-3) AC | sc-137128 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CSP Antibody (H-3) HRP | sc-137128 HRP | 200 µg/ml | $316.00 | |||
CSP Antibody (H-3) FITC | sc-137128 FITC | 200 µg/ml | $330.00 | |||
CSP Antibody (H-3) PE | sc-137128 PE | 200 µg/ml | $343.00 | |||
CSP Antibody (H-3) Alexa Fluor® 488 | sc-137128 AF488 | 200 µg/ml | $357.00 | |||
CSP Antibody (H-3) Alexa Fluor® 546 | sc-137128 AF546 | 200 µg/ml | $357.00 | |||
CSP Antibody (H-3) Alexa Fluor® 594 | sc-137128 AF594 | 200 µg/ml | $357.00 | |||
CSP Antibody (H-3) Alexa Fluor® 647 | sc-137128 AF647 | 200 µg/ml | $357.00 | |||
CSP Antibody (H-3) Alexa Fluor® 680 | sc-137128 AF680 | 200 µg/ml | $357.00 | |||
CSP Antibody (H-3) Alexa Fluor® 790 | sc-137128 AF790 | 200 µg/ml | $357.00 |