cSHMT Antibody (F-9): sc-514410

cSHMT Antibody (F-9) is a mouse monoclonal IgG1 antibody that detects serine hydroxymethyltransferase (cSHMT) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). cSHMT is a crucial enzyme that plays a significant role in one-carbon metabolism, catalyzing the reversible conversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in both the cytoplasm and mitochondria. This enzymatic activity is vital for the synthesis of nucleotides, particularly thymidylate, which is essential for DNA replication and repair. Anti-cSHMT antibody (F-9) recognizes an enzyme critical for cellular proliferation and overall metabolic health, as cSHMT helps maintain the balance of one-carbon units necessary for various biosynthetic pathways. The enzyme′s tetrameric structure is stabilized by specific residues, such as Lys-71, Arg-80, and Asp-89, which are essential for catalytic activity, while the active site residues, including Tyr-72, Asp-227, and His-356, interact with pyridoxal phosphate, ensuring efficient substrate conversion. The human cSHMT gene is located on chromosome 17p11.2, and the mitochondrial variant is encoded by a gene on chromosome 12q13, with the cDNA for the mitochondrial enzyme producing a mature protein of 474 residues. cSHMT monoclonal antibody (F-9) serves as a valuable tool for researchers studying one-carbon metabolism and its implications in health and disease.

The preferred cSHMT antibody is cSHMT Antibody (A-2): sc-365203.