CRY2 Antibody (3H4): sc-293263

CRY2 Antibody (3H4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CRY2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-CRY2 antibody (3H4) is available as the non-conjugated form. CRY2 plays a crucial role in regulating circadian rhythms, acting as a light-independent inhibitor of the circadian clock. CRY2 is primarily located in the nucleus of cells, where CRY2 interacts with key transcription factors such as Clock and BMAL1, which are essential for generating and maintaining circadian rhythms. By forming heterodimers with other proteins like Per1 and Per2, CRY2 helps modulate the activity of these transcription factors, thereby influencing gene expression involved in light/dark cycle entrainment. This regulation is vital for maintaining the body′s internal clock, which affects various physiological processes, including sleep-wake cycles, hormonal secretions, and metabolic functions. CRY2′s ability to inhibit Clock-BMAL1 dimers underscores CRY2′s importance in feedback mechanisms that sustain circadian rhythms, making CRY2 a significant target for studies related to sleep disorders and metabolic diseases.

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CRY2 Antibody (3H4) References:

1. Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2.  |  Vitaterna, MH., et al. 1999. Proc Natl Acad Sci U S A. 96: 12114-9. PMID: 10518585
2. Light-independent role of CRY1 and CRY2 in the mammalian circadian clock.  |  Griffin, EA., et al. 1999. Science. 286: 768-71. PMID: 10531061
3. The CRY2-COP1-HY5-BBX7/8 module regulates blue light-dependent cold acclimation in Arabidopsis.  |  Li, Y., et al. 2021. Plant Cell. 33: 3555-3573. PMID: 34427646
4. CRY2 isoform selectivity of a circadian clock modulator with antiglioblastoma efficacy.  |  Miller, S., et al. 2022. Proc Natl Acad Sci U S A. 119: e2203936119. PMID: 36161947
5. Light-induced LLPS of the CRY2/SPA1/FIO1 complex regulating mRNA methylation and chlorophyll homeostasis in Arabidopsis.  |  Jiang, B., et al. 2023. Nat Plants. 9: 2042-2058. PMID: 38066290
6. The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth.  |  Zeng, D., et al. 2025. Cell. 188: 60-76.e20. PMID: 39549699
7. A 24-hour circadian clock is found in the mammalian retina.  |  Morell, V. 1996. Science. 272: 349. PMID: 8602520
8. A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light.  |  Albrecht, U., et al. 1997. Cell. 91: 1055-64. PMID: 9428527
9. Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription.  |  Sangoram, AM., et al. 1998. Neuron. 21: 1101-13. PMID: 9856465
10. Molecular analysis of mammalian timeless.  |  Zylka, MJ., et al. 1998. Neuron. 21: 1115-22. PMID: 9856466
11. Molecular bases for circadian clocks.  |  Dunlap, JC. 1999. Cell. 96: 271-90. PMID: 9988221
12. A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock.  |  Jin, X., et al. 1999. Cell. 96: 57-68. PMID: 9989497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRY2 Antibody (3H4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CRY2 Antibody (3H4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRY2 Antibody (3H4) Specifications

Alternate Product Name: CRY2

Product Clone Name: 3H4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 141-231

Epitope Target: CRY2

Epitope Species: human

Product Reactivity/Detection: CRY2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CRY2 Antibody (3H4): N/A