CRTAP Antibody (E-1): sc-393136
- CRTAP Antibody (E-1) is a mouse monoclonal IgG1 κ CRTAP antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 171-255 mapping within an internal region of CRTAP of human origin
- CRTAP Antibody (E-1) is recommended for detection of CRTAP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CRTAP Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CRTAP Antibody (E-1) for WB applications. These reagents are now offered in bundles with CRTAP Antibody (E-1) (see ordering information below).
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CRTAP Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRTAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRTAP (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRTAP protein, also known as cartilage associated protein, CASP, or leprecan-like 3 (LEPREL3), is a secreted protein primarily located in the extracellular space, where CRTAP plays a crucial role in collagen post-translational modifications, extracellular fibril assembly, and intracellular trafficking. CRTAP is predominantly expressed in articular chondrocytes and is essential for normal bone formation. CRTAP contains a signal peptide and a tetratricopeptide-like helical domain, which are important for CRTAP function in the endoplasmic reticulum. Within this cellular compartment, CRTAP forms a complex with Gros1 and cyclophilin B, which is vital for the efficient 3-hydroxylation of prolyl residues in collagen type I molecules, the major structural proteins of skin and bone. Deficiencies or mutations in the gene encoding CRTAP can lead to autosomal recessive osteogenesis imperfecta (OI) types 7 and 2B, conditions characterized by bone fragility and increased susceptibility to fractures. OI type 7 is a milder form of the disorder, while OI type 2B is associated with a neonatal lethal phenotype, underscoring the importance of CRTAP in skeletal integrity and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CRTAP Antibody (E-1) References:
- cDNA cloning, characterization and chromosome mapping of Crtap encoding the mouse cartilage associated protein. | Morello, R., et al. 1999. Matrix Biol. 18: 319-24. PMID: 10429950
- cDNA cloning, characterization and chromosome mapping of the gene encoding human cartilage associated protein (CRTAP). | Tonachini, L., et al. 1999. Cytogenet Cell Genet. 87: 191-4. PMID: 10702664
- CRTAP is required for prolyl 3- hydroxylation and mutations cause recessive osteogenesis imperfecta. | Morello, R., et al. 2006. Cell. 127: 291-304. PMID: 17055431
- Deficiency of cartilage-associated protein in recessive lethal osteogenesis imperfecta. | Barnes, AM., et al. 2006. N Engl J Med. 355: 2757-64. PMID: 17192541
- Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta. | Cabral, WA., et al. 2007. Nat Genet. 39: 359-65. PMID: 17277775
- Components of the collagen prolyl 3-hydroxylation complex are crucial for normal bone development. | Marini, JC., et al. 2007. Cell Cycle. 6: 1675-81. PMID: 17630507
- Heritability of alternative splicing in the human genome. | Kwan, T., et al. 2007. Genome Res. 17: 1210-8. PMID: 17671095
- Osteogenesis imperfecta:epidemiology and pathophysiology. | Martin, E. and Shapiro, JR. 2007. Curr Osteoporos Rep. 5: 91-7. PMID: 17925189
- Cartilage associated protein (CASP) is a novel developmentally regulated chick embryo protein. | Castagnola, P., et al. 1997. J Cell Sci. 110 (Pt 12): 1351-9. PMID: 9217321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRTAP Antibody (E-1) | sc-393136 | 200 µg/ml | $322.00 | |||
CRTAP Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-530367 | 200 µg Ab; 10 µg BP | $361.00 | |||
CRTAP Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-523820 | 200 µg Ab, 40 µg BP | $361.00 | |||
CRTAP Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-544018 | 200 µg Ab; 20 µg BP | $361.00 | |||
CRTAP Antibody (E-1) AC | sc-393136 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CRTAP Antibody (E-1) HRP | sc-393136 HRP | 200 µg/ml | $322.00 | |||
CRTAP Antibody (E-1) FITC | sc-393136 FITC | 200 µg/ml | $336.00 | |||
CRTAP Antibody (E-1) PE | sc-393136 PE | 200 µg/ml | $349.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 488 | sc-393136 AF488 | 200 µg/ml | $364.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 546 | sc-393136 AF546 | 200 µg/ml | $364.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 594 | sc-393136 AF594 | 200 µg/ml | $364.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 647 | sc-393136 AF647 | 200 µg/ml | $364.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 680 | sc-393136 AF680 | 200 µg/ml | $364.00 | |||
CRTAP Antibody (E-1) Alexa Fluor® 790 | sc-393136 AF790 | 200 µg/ml | $364.00 |