CRSP77 Antibody (2215C3a): sc-81238
- CRSP77 Antibody (2215C3a) is a mouse monoclonal IgG1 CRSP77 antibody provided at 100 µg/ml
- raised against a recombinant protein corresponding to an internal region of CRSP77 of human origin
- recommended for detection of CRSP77 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CRSP77 Antibody (2215C3a). This work is in progress.
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CRSP77 Antibody (2215C3a) is a mouse monoclonal IgG1 antibody that detects CRSP77 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-CRSP77 antibody (2215C3a) is available as the non-conjugated format. CRSP77 protein plays a crucial role in transcription regulation within mammalian cells, functioning as part of the CRSP (cofactor required for Sp1 activation) complex, which mediates signals between transcriptional activators and RNA polymerase. CRSP77 protein is part of a multimeric complex consisting of nine distinct subunits, with ubiquitous expression across various tissues highlighting importance in gene regulation. CRSP77 (2215C3a) antibody recognizes a protein that, along with associated subunits, interacts with critical components of transcription machinery, including TAFIIs, necessary for transcriptional activation by Sp1. CRSP77 protein relates to other proteins within the murine mediator complex, emphasizing an integral role in transcriptional regulation. Understanding CRSP77 structure and function is vital, as CRSP77 protein facilitates specific gene transcription and interacts with larger coactivator complexes, influencing various biological processes.
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CRSP77 Antibody (2215C3a) References:
- Composite co-activator ARC mediates chromatin-directed transcriptional activation. | Näär, AM., et al. 1999. Nature. 398: 828-32. PMID: 10235267
- Purification of transcription cofactor complex CRSP. | Ryu, S. and Tjian, R. 1999. Proc Natl Acad Sci U S A. 96: 7137-42. PMID: 10377381
- Three-dimensional structure of the human TFIID-IIA-IIB complex. | Andel, F., et al. 1999. Science. 286: 2153-6. PMID: 10591646
- Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation. | Näär, AM., et al. 2002. Genes Dev. 16: 1339-44. PMID: 12050112
- A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. | Kim, YJ., et al. 1994. Cell. 77: 599-608. PMID: 8187178
- The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. | Myers, LC., et al. 1998. Genes Dev. 12: 45-54. PMID: 9420330
- Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. | Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
- The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. | Ryu, S., et al. 1999. Nature. 397: 446-50. PMID: 9989412
- Purification of transcription cofactor complex CRSP | . 1999. Biochem. 96: 7137-7142.
- Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator | . 2002. Science. 295: 1058-1062.
- Structure and Function of CRSP/Med2 A Promoter-Selective Transcriptional Coactivator Complex | . 2004. Mol Cell. 14: 675-683.
- Distinct conformational states of nuclear receptor–bound CRSP–Med complexes | . 2004. Nat Struct Mol Biol. 11: 664–671.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRSP77 Antibody (2215C3a) | sc-81238 | 100 µg/ml | $333.00 |