CRSP70 Antibody (F-5): sc-137196

CRSP70 Antibody (F-5) is a mouse monoclonal IgG2b antibody that detects CRSP70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRSP70, a crucial component of the CRSP (cofactor required for Sp1 activation) complex, plays a significant role in the regulation of transcription by facilitating the interaction between transcriptional activators and RNA polymerase. CRSP70 is primarily located in the nucleus, where CRSP70 is essential for the assembly of transcriptional machinery and the modulation of gene expression. Nuclear localization of CRSP70 allows for precise regulation of transcriptional responses to various cellular signals, ensuring genes are expressed at the right time and in the right amounts. CRSP70′s interaction with other subunits of the CRSP complex and larger coactivator complexes, such as ARC/DRI, underscores CRSP70′s importance in mediating transcriptional activation of genes regulated by nuclear hormone receptors and other transcription factors. Using anti-CRSP70 antibody (F-5), researchers can gain insights into the dynamics of transcriptional regulation and the intricate networks that govern gene expression in various biological contexts.

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CRSP70 Antibody (F-5) References:

1. Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70.  |  Booth, V., et al. 2000. J Biol Chem. 275: 31266-8. PMID: 10811649
2. MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.  |  Ding, N., et al. 2009. J Biol Chem. 284: 2648-2656. PMID: 19049968
3. The structure of an Iws1/Spt6 complex reveals an interaction domain conserved in TFIIS, Elongin A and Med26.  |  Diebold, ML., et al. 2010. EMBO J. 29: 3979-91. PMID: 21057455
4. Human mediator subunit MED26 functions as a docking site for transcription elongation factors.  |  Takahashi, H., et al. 2011. Cell. 146: 92-104. PMID: 21729782
5. MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.  |  Takahashi, H., et al. 2015. Nat Commun. 6: 5941. PMID: 25575120
6. 1H, 15N and 13C assignments of the N-terminal domain of the Mediator complex subunit MED26.  |  Peruzzini, R., et al. 2016. Biomol NMR Assign. 10: 233-6. PMID: 26861138
7. Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.  |  Lens, Z., et al. 2017. J Mol Biol. 429: 3043-3055. PMID: 28893534
8. The role of Mediator and Little Elongation Complex in transcription termination.  |  Takahashi, H., et al. 2020. Nat Commun. 11: 1063. PMID: 32102997
9. A simple protocol to purify human TFIID free of the MED26 subunit of mediator complex.  |  Xu, M. and Martinez, E. 2021. Protein Expr Purif. 184: 105887. PMID: 33836240
10. MED26-containing Mediator may orchestrate multiple transcription processes through organization of nuclear bodies.  |  Suzuki, H., et al. 2023. Bioessays. 45: e2200178. PMID: 36852638

The preferred CRSP70 antibody is CRSP70 Antibody (G-11): sc-166614.