CRP1 Antibody (F-12): sc-390418
- CRP1 Antibody (F-12) is a mouse monoclonal IgG1 κ CRP1 antibody provided at 200 µg/ml
- raised against amino acids 8-96 mapping near the N-terminus of CRP1 of human origin
- CRP1 Antibody (F-12) is recommended for detection of CRP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CRP1 Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CRP1 Antibody (F-12) for WB and IHC(P) applications. These reagents are now offered in bundles with CRP1 Antibody (F-12) (see ordering information below).
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SEE ALSO...
CRP1 Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CRP1 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRP1, also known as cysteine and glycine-rich protein 1, plays a crucial role in organizing multiprotein complexes, particularly in the cytoplasm and nucleus, where CRP1 is involved in cytoskeletal remodeling and smooth muscle differentiation. CRP1 contains two LIM zinc-binding domains that are essential for interaction with adhesion-plaque protein Zyxin, as well as with α-actinin, which is vital for muscle differentiation. These interactions highlight CRP1′s structural function, emphasizing CRP1′s importance in maintaining cellular architecture and facilitating muscle development. CRP1′s abundance in prostate and smooth muscle lineages further underscores CRP1′s significance in tissue-specific functions and cellular integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CRP1 Antibody (F-12) References:
- The LIM protein, CRP1, is a smooth muscle marker. | Henderson, JR., et al. 1999. Dev Dyn. 214: 229-38. PMID: 10090149
- Cysteine-rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors. | Chang, DF., et al. 2003. Dev Cell. 4: 107-18. PMID: 12530967
- The cysteine-rich protein family of highly related LIM domain proteins. | Weiskirchen, R., et al. 1995. J Biol Chem. 270: 28946-54. PMID: 7499425
- CRP1, a LIM domain protein implicated in muscle differentiation, interacts with alpha-actinin. | Pomiès, P., et al. 1997. J Cell Biol. 139: 157-68. PMID: 9314536
- Abundant cysteine-rich protein-1 is localized in the stromal compartment of the human prostate. | Dubé, JY., et al. 1998. Arch Androl. 40: 109-15. PMID: 9507743
- LIM domains of cysteine-rich protein 1 (CRP1) are essential for its zyxin-binding function. | Schmeichel, KL. and Beckerle, MC. 1998. Biochem J. 331 (Pt 3): 885-92. PMID: 9560318
- Assignment1 of CSRP1 encoding the LIM domain protein CRP1, to human chromosome 1q32 by fluorescence in situ hybridization. | Erdel, M. and Weiskirchen, R. 1998. Cytogenet Cell Genet. 83: 10-1. PMID: 9925910
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRP1 Antibody (F-12) | sc-390418 | 200 µg/ml | $322.00 | |||
CRP1 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-530153 | 200 µg Ab; 10 µg BP | $361.00 | |||
CRP1 Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-523529 | 200 µg Ab, 40 µg BP | $361.00 | |||
CRP1 Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-543896 | 200 µg Ab; 20 µg BP | $361.00 | |||
CRP1 Antibody (F-12) AC | sc-390418 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CRP1 Antibody (F-12) HRP | sc-390418 HRP | 200 µg/ml | $322.00 | |||
CRP1 Antibody (F-12) FITC | sc-390418 FITC | 200 µg/ml | $336.00 | |||
CRP1 Antibody (F-12) PE | sc-390418 PE | 200 µg/ml | $349.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 488 | sc-390418 AF488 | 200 µg/ml | $364.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 546 | sc-390418 AF546 | 200 µg/ml | $364.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 594 | sc-390418 AF594 | 200 µg/ml | $364.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 647 | sc-390418 AF647 | 200 µg/ml | $364.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 680 | sc-390418 AF680 | 200 µg/ml | $364.00 | |||
CRP1 Antibody (F-12) Alexa Fluor® 790 | sc-390418 AF790 | 200 µg/ml | $364.00 |