Cripto Antibody (H-10): sc-376448

Cripto Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cripto protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Cripto antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cripto protein, also known as cripto-1, is encoded by the teratocarcinoma-derived growth factor (TDGF)-1 gene and plays a crucial role in early embryonic development, particularly during gastrulation when Cripto is first expressed in the forming mesoderm. Cripto expression later becomes restricted to the truncus arteriosus of the developing heart, indicating Cripto′s involvement in the progression of epiblastic cells that contribute to mesoderm formation. In adult tissues, Cripto is found at low levels in the spleen, heart, lung, and brain, but Cripto overexpression is notably associated with human gastric and colorectal carcinomas, highlighting Cripto′s potential as a biomarker for cancer progression and a target for therapeutic intervention.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cripto Antibody (H-10) References:

1. A truncated form of teratocarcinoma-derived growth factor-1 (cripto-1) mRNA expressed in human colon carcinoma cell lines and tumors.  |  Baldassarre, G., et al. 2001. Tumour Biol. 22: 286-93. PMID: 11553858
2. Cripto: a tumor growth factor and more.  |  Adamson, ED., et al. 2002. J Cell Physiol. 190: 267-78. PMID: 11857442
3. Cripto forms a complex with activin and type II activin receptors and can block activin signaling.  |  Gray, PC., et al. 2003. Proc Natl Acad Sci U S A. 100: 5193-8. PMID: 12682303
4. Decrypting the role of Cripto in tumorigenesis.  |  Shen, MM. 2003. J Clin Invest. 112: 500-2. PMID: 12925690
5. Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells.  |  Parisi, S., et al. 2003. J Cell Biol. 163: 303-14. PMID: 14581455
6. Cripto: a novel target for antibody-based cancer immunotherapy.  |  Xing, PX., et al. 2004. Cancer Res. 64: 4018-23. PMID: 15173016
7. Human Cripto-1 as a target for a cancer vaccine: WO2008040759.  |  Bianco, C. and Salomon, DS. 2009. Expert Opin Ther Pat. 19: 141-4. PMID: 19441915
8. Cripto-1: an embryonic gene that promotes tumorigenesis.  |  de Castro, NP., et al. 2010. Future Oncol. 6: 1127-42. PMID: 20624125
9. Targeting Nodal and Cripto-1: Perspectives Inside Dual Potential Theranostic Cancer Biomarkers.  |  Sandomenico, A. and Ruvo, M. 2019. Curr Med Chem. 26: 1994-2050. PMID: 30207211
10. Cripto-1 as a Key Factor in Tumor Progression, Epithelial to Mesenchymal Transition and Cancer Stem Cells.  |  Arnouk, H., et al. 2021. Int J Mol Sci. 22: PMID: 34502188
11. The murine cripto gene: expression during mesoderm induction and early heart morphogenesis.  |  Dono, R., et al. 1993. Development. 118: 1157-68. PMID: 7916676
12. Identification and biological characterization of an epidermal growth factor-related protein: cripto-1.  |  Brandt, R., et al. 1994. J Biol Chem. 269: 17320-8. PMID: 8006041

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cripto Antibody (H-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Cripto Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cripto Antibody (H-10) Specifications

Alternate Product Name: Cripto

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-188

Epitope Target: Cripto

Epitope Species: human

Product Reactivity/Detection: Cripto

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Cripto Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin