CRHSP-24 Antibody (A-1): sc-137072

CRHSP-24 Antibody (A-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects CRHSP-24 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CRHSP-24 (A-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRHSP-24, also known as calcium-regulated heat-stable protein 24, is a serine phosphoprotein that plays a crucial role in calcium-mediated signal transduction, primarily due to phosphorylation on serine residues. CRHSP-24 is ubiquitously expressed and has been identified as a physiological substrate for Ca2+-calmodulin regulated protein phosphatase calcineurin (PP2B), highlighting CRHSP-24′s importance in cellular signaling pathways. CRHSP-24 interacts with STYX/dead phosphate protein during spermatid development and is a paralog of brain-specific mRNA-binding protein PIPPIN. Phosphorylation of CRHSP-24 on Ser 52 by Akt-1 (PKBα) occurs in response to insulin-like growth factor 1 (IGF-1) and by both Akt-1 and ribosomal S6 kinase (RSK) in response to epidermal growth factor (EGF). Additionally, phosphorylation on Ser 41 in the absence of IGF-1/EGF by a DYRK isoform or another proline-directed protein kinase further illustrates CRHSP-24′s complex regulatory mechanisms.

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CRHSP-24 Antibody (A-1) References:

1. Intracellular signaling mechanisms activated by cholecystokinin-regulating synthesis and secretion of digestive enzymes in pancreatic acinar cells.  |  Williams, JA. 2001. Annu Rev Physiol. 63: 77-97. PMID: 11181949
2. PTEN and myotubularin phosphatases: from 3-phosphoinositide dephosphorylation to disease.  |  Wishart, MJ. and Dixon, JE. 2002. Trends Cell Biol. 12: 579-85. PMID: 12495846
3. CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells.  |  Schäfer, C., et al. 2003. Am J Physiol Gastrointest Liver Physiol. 285: G726-34. PMID: 12801884
4. Calcineurin mediates pancreatic growth in protease inhibitor-treated mice.  |  Tashiro, M., et al. 2004. Am J Physiol Gastrointest Liver Physiol. 286: G784-90. PMID: 14684381
5. Calcineurin is required for translational control of protein synthesis in rat pancreatic acini.  |  Sans, MD. and Williams, JA. 2004. Am J Physiol Cell Physiol. 287: C310-9. PMID: 15044154
6. Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL.  |  Auld, GC., et al. 2005. Biochem J. 389: 775-83. PMID: 15910284
7. Intracellular regulatory mechanisms in pancreatic acinar cellular function.  |  Williams, JA. 1999. Curr Opin Gastroenterol. 15: 385-91. PMID: 17023978
8. Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini.  |  Burnham, DB. and Williams, JA. 1982. J Biol Chem. 257: 10523-8. PMID: 7050109
9. Purification and characterization of a novel physiological substrate for calcineurin in mammalian cells.  |  Groblewski, GE., et al. 1998. J Biol Chem. 273: 22738-44. PMID: 9712905

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRHSP-24 Antibody (A-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CRHSP-24 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRHSP-24 Antibody (A-1) Specifications

Alternate Product Name: CRHSP-24

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 9-53

Epitope Target: CRHSP-24

Epitope Species: human

Product Reactivity/Detection: CRHSP-24

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CRHSP-24 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin