Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRELD2 CRISPR/Cas9 KO Plasmid (h): sc-407369
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRELD2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRELD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRELD2 Antibody (A-8): sc-365168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRELD2 CRISPR/Cas9 KO Plasmid (h) | sc-407369 | 20 µg | $397.00 |
Overview
CRELD2 (cysteine rich with EGF like domains 2) encodes an endoplasmic reticulum–resident, disulfide bond–containing protein implicated in protein quality control and secretory pathway homeostasis. CRELD2 expression is commonly induced during ER stress and is linked to unfolded protein response signaling, where it can influence proteostasis, redox balance, and secretion of client proteins. Through these processes, CRELD2 has been studied in contexts of cellular stress adaptation and inflammation-related signaling, and altered expression has been reported across multiple disease-relevant settings including cancer and metabolic or fibrotic phenotypes. As a modulator of ER-associated pathways, CRELD2 provides a tractable node for dissecting how stress-responsive secretory programs shape cell state and survival.
CRELD2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CRELD2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CRELD2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CRELD2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRELD2 protein expression.
This CRISPR knockout system enables efficient generation of CRELD2-deficient cell models for investigation of CRELD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CRELD2 exon(s) critical for CRELD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CRELD2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRELD2 CRISPR/Cas9 KO Plasmid (h) and CRELD2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CRELD2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRELD2 HDR Plasmid (h) and CRELD2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CRELD2 homology arms to support homology-directed repair at defined CRELD2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.