creatine kinase-M Antibody (H-9): sc-365141

Creatine kinase-M Antibody (H-9) is a mouse monoclonal IgG3 antibody that detects creatine kinase-M in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Creatine kinase-M, a muscle tissue-specific isoform of creatine kinase, plays a crucial role in energy metabolism by catalyzing the reversible transfer of phosphate from creatine to adenosine diphosphate (ADP), thereby generating adenosine triphosphate (ATP) during periods of high energy demand. This function is particularly important in muscle cells, where ATP is essential for muscle contraction and overall cellular function. Creatine kinase-M consists of 381 amino acids and is predominantly found in skeletal muscle, making creatine kinase-M a key player in maintaining energy homeostasis in muscle tissues. Additionally, creatine kinase-M is involved in the regulation of calcium homeostasis, which is vital for muscle contraction and relaxation. The ability of creatine kinase-M to rapidly regenerate ATP ensures that muscle cells can respond effectively to fluctuating energy demands, highlighting creatine kinase-M′s significance in both normal physiology and in conditions such as muscle injury or disease. Anti-creatine kinase-M antibody (H-9) is an invaluable tool for researchers studying muscle metabolism and related pathologies.

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creatine kinase-M Antibody (H-9) References:

1. [Clinical significance of the change of serum CK-MM in electrical injured patients].  |  Liu, S. and Xie, W. 2002. Zhonghua Shao Shang Za Zhi. 18: 226-8. PMID: 12467535
2. [Immunoinhibitory determination of CK-MM subbands by monoclonal antibody].  |  Okabe, H., et al. 1991. Rinsho Byori. 39: 1140-6. PMID: 1762189
3. CK-MM PGAM-MM G6PD and am biochemical markers of functional innervated cultured human muscle fibers.  |  Meola, G., et al. 1991. Cytotechnology. 5: 176-7. PMID: 22358988
4. [Immunoelectron microscopic studies on the localization of creatine kinase M in normal and ischemic myocardial cells].  |  Otsu, N., et al. 1990. Rinsho Byori. 38: 773-81. PMID: 2402081
5. Post synthetic modification of CK-MM by kininase I.  |  van Sande, M., et al. 1989. Adv Exp Med Biol. 247A: 319-24. PMID: 2603797
6. Aggregation of cysteamine-capped gold nanoparticles in presence of ATP as an analytical tool for rapid detection of creatine kinase (CK-MM).  |  Sharma, AK., et al. 2018. Anal Chim Acta. 1024: 161-168. PMID: 29776542
7. Creatine kinase isoenzyme profiles after exercise in the rat: sex-linked differences in leakage of CK-MM.  |  Amelink, GJ., et al. 1988. Pflugers Arch. 412: 417-21. PMID: 3174399
8. Ischemia induces changes in the level of mRNAs coding for stress protein 71 and creatine kinase M.  |  Mehta, HB., et al. 1988. Circ Res. 63: 512-7. PMID: 3409483
9. Early diagnosis of acute myocardial infarction by rapid analysis of creatine kinase isoenzyme-3 (CK-MM) sub-types.  |  Wu, AH., et al. 1987. Clin Chem. 33: 358-62. PMID: 3815799
10. Improved quantification with validation of multiple mRNA species by polymerase chain reaction: application to human myocardial creatine kinase M and B.  |  Ma, TS., et al. 1994. Cardiovasc Res. 28: 464-71. PMID: 8181032

The preferred creatine kinase-M antibody is creatine kinase-M Antibody (G-9): sc-365046.