creatine kinase-M Antibody (B-1): sc-390044

Creatine kinase-M Antibody (B-1) is a mouse monoclonal IgG3 antibody that detects creatine kinase-M in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Creatine kinase-M, a muscle tissue-specific isoform of creatine kinase, plays a crucial role in energy metabolism by catalyzing the reversible transfer of phosphate from creatine to adenosine diphosphate (ADP), thereby generating adenosine triphosphate (ATP) during periods of high energy demand. This function is particularly important in muscle cells, where ATP is essential for muscle contraction and overall cellular function. Creatine kinase-M consists of 381 amino acids and is predominantly found in skeletal muscle, making creatine kinase-M a key player in maintaining energy homeostasis in muscle tissues. Additionally, creatine kinase-M is involved in the regulation of calcium homeostasis, which is vital for muscle contraction and relaxation. The ability of creatine kinase-M to rapidly regenerate ATP ensures that muscle cells can respond effectively to fluctuating energy demands, highlighting creatine kinase-M′s significance in both normal physiology and in conditions such as muscle injury or disease. Anti-creatine kinase-M antibody (B-1) is an invaluable tool for researchers studying muscle metabolism and related pathologies, providing insights into the intricate mechanisms of energy regulation in muscle tissues.

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creatine kinase-M Antibody (B-1) References:

1. Alterations in the expression and activity of creatine kinase-M and mitochondrial creatine kinase subunits in skeletal muscle following prolonged intense exercise in rats.  |  Chen, Y., et al. 2000. Eur J Appl Physiol. 81: 114-9. PMID: 10552275
2. Direct haplotyping by double digestion of PCR-amplified creatine kinase (CKMM): application to myotonic dystrophy diagnosis.  |  Lavedan, C., et al. 1990. Genomics. 8: 739-40. PMID: 1980487
3. Tight linkage of creatine kinase (CKMM) to myotonic dystrophy on chromosome 19.  |  Yamaoka, LH., et al. 1990. Neurology. 40: 222-6. PMID: 2300239
4. A long-range restriction map of the human chromosome 19q13 region: close physical linkage between CKMM and the ERCC1 and ERCC2 genes.  |  Smeets, H., et al. 1990. Am J Hum Genet. 46: 492-501. PMID: 2309701
5. Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa.  |  Huszar, G. and Vigue, L. 1990. Mol Reprod Dev. 25: 258-62. PMID: 2331374
6. Appearance of M-band attached MM-creatine kinase in differentiating chicken heart cells after injection of M-type isoprotein or poly (A+)-RNA enriched for M-type creatine kinase message.  |  Schäfer, B., et al. 1985. Basic Res Cardiol. 80 Suppl 2: 129-33. PMID: 2415105
7. Myotonic dystrophy is closely linked to the gene for muscle-type creatine kinase (CKMM).  |  Brunner, HG., et al. 1989. Hum Genet. 81: 308-10. PMID: 2703233
8. The Best of Both Worlds: Eliminating Creatine Kinase-Muscle/Brain (CK-MB) Testing in the Emergency Department Leads to Lower Costs Without Missed Clinical Diagnoses.  |  Sahadeo, PA., et al. 2021. Cureus. 13: e15150. PMID: 34164248
9. Expression of creatine kinase M and B mRNAs in treadmill trained rat skeletal muscle.  |  Apple, FS. and Billadello, JJ. 1994. Life Sci. 55: 585-92. PMID: 8046994
10. Different regulatory sequences control creatine kinase-M gene expression in directly injected skeletal and cardiac muscle.  |  Vincent, CK., et al. 1993. Mol Cell Biol. 13: 1264-72. PMID: 8423791

The preferred creatine kinase-M antibody is creatine kinase-M Antibody (G-9): sc-365046.