CPTI Antibody (E-7): sc-393070

CPTI Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPTI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CPTI antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPTI plays a crucial role in mitochondrial β-oxidation of long-chain fatty acids, initiating fatty acid transport from cytoplasm into mitochondrion, which is a rate-limiting step in the metabolic pathway. This process is essential for energy production, particularly in tissues that rely heavily on fatty acid oxidation, such as heart and skeletal muscle. CPTI exists in two isoforms: CPTIA, predominantly expressed in liver, and CPTIB, specifically found in muscle tissues. Membrane-bound CPTI is reversibly inhibited by malonyl-coenzyme A, highlighting regulatory mechanisms that control fatty acid metabolism. Specific amino acids, such as glutamic acid at position 3 and histidine at position 5, are critical for malonyl CoA binding and inhibition in liver CPTI, although these residues do not affect catalytic activity. This intricate regulation underscores CPTI′s importance in maintaining metabolic homeostasis and energy balance within cells.

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CPTI Antibody (E-7) References:

1. Functional characterization of mammalian mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris.  |  Woldegiorgis, G., et al. 2000. J Nutr. 130: 310S-314S. PMID: 10721894
2. Pig muscle carnitine palmitoyltransferase I (CPTI beta), with low Km for carnitine and low sensitivity to malonyl-CoA inhibition, has kinetic characteristics similar to those of the rat liver (CPTI alpha) enzyme.  |  Relat, J., et al. 2004. Biochemistry. 43: 12686-91. PMID: 15449958
3. Limited fitness advantages of crop-weed hybrid progeny containing insect-resistant transgenes (Bt/CpTI) in transgenic rice field.  |  Yang, X., et al. 2012. PLoS One. 7: e41220. PMID: 22815975
4. Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system.  |  Woeltje, KF., et al. 1990. J Biol Chem. 265: 10714-9. PMID: 2355017
5. Regulation of ketogenesis and the renaissance of carnitine palmitoyltransferase.  |  McGarry, JD., et al. 1989. Diabetes Metab Rev. 5: 271-84. PMID: 2656156
6. Surface treatment influences electrochemical stability of cpTi exposed to mouthwashes.  |  Beline, T., et al. 2016. Mater Sci Eng C Mater Biol Appl. 59: 1079-1088. PMID: 26652467
7. Enhanced osteogenesis and bactericidal performance with additively manufactured MgO and Cu-added CpTi for load-bearing implants.  |  Ciliveri, S. and Bandyopadhyay, A. 2023. Int J Bioprint. 9: PMID: 38116397
8. Human liver mitochondrial carnitine palmitoyltransferase I: characterization of its cDNA and chromosomal localization and partial analysis of the gene.  |  Britton, CH., et al. 1995. Proc Natl Acad Sci U S A. 92: 1984-8. PMID: 7892212
9. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I.  |  Yamazaki, N., et al. 1996. Biochim Biophys Acta. 1307: 157-61. PMID: 8679700
10. Structural features of the gene encoding human muscle type carnitine palmitoyltransferase I.  |  Yamazaki, N., et al. 1997. FEBS Lett. 409: 401-6. PMID: 9224698
11. Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I.  |  Zhu, H., et al. 1997. Arch Biochem Biophys. 347: 53-61. PMID: 9344464
12. Characterization of carnitine acylcarnitine translocase system of heart mitochondria.  |  Pande, SV. and Parvin, R. 1976. J Biol Chem. 251: 6683-91. PMID: 977593

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPTI Antibody (E-7) has 40 citations in a variety of scientific publications.

Primary antibodies like Anti-CPTI Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPTI Antibody (E-7) Specifications

Alternate Product Name: CPT1A / CPT1B

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 665-688

Epitope Target: CPTI

Epitope Species: human

Product Reactivity/Detection: CPTI, liver isoform (CPTI-L) and muscle isoform (CPTI-M)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 40 citations in various scientific publications

Available Conjugates/Forms for CPTI Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin