Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CPSF4 CRISPR/Cas9 KO Plasmid (h): sc-403190
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPSF4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CPSF4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPSF4 Antibody (A-11): sc-393316
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF4 CRISPR/Cas9 KO Plasmid (h) | sc-403190 | 20 µg | $397.00 |
Overview
CPSF4 (cleavage and polyadenylation specificity factor subunit 4) encodes a core component of the mRNA 3′ end processing machinery that recognizes polyadenylation signals and coordinates cleavage and poly(A) tail addition. Through its role in the cleavage and polyadenylation complex, CPSF4 helps regulate transcript maturation, alternative polyadenylation, and gene expression output, impacting mRNA stability and translation. Perturbation of 3′ end processing factors can shift poly(A) site usage and broadly reprogram transcriptional networks, linking CPSF4-associated biology to cell-cycle control, stress responses, and differentiation programs. Dysregulated RNA processing and alternative polyadenylation are frequently observed in cancer and other proliferative or neurodevelopmental contexts, making CPSF4 a relevant node for mechanistic studies.
CPSF4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CPSF4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CPSF4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CPSF4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CPSF4 protein expression.
This CRISPR knockout system enables efficient generation of CPSF4-deficient cell models for investigation of CPSF4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CPSF4 exon(s) critical for CPSF4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CPSF4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CPSF4 CRISPR/Cas9 KO Plasmid (h) and CPSF4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CPSF4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CPSF4 HDR Plasmid (h) and CPSF4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CPSF4 homology arms to support homology-directed repair at defined CPSF4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.