Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CPSF2 CRISPR/Cas9 KO Plasmid (h): sc-407012
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPSF2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CPSF2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CPSF2 Antibody (A-11): sc-165983
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF2 CRISPR/Cas9 KO Plasmid (h) | sc-407012 | 20 µg | $397.00 |
Overview
CPSF2 (cleavage and polyadenylation specificity factor subunit 2) is a core component of the mammalian 3′ end mRNA processing machinery that contributes to recognition of polyadenylation signals and assembly of the cleavage and polyadenylation complex. Through its role in pre-mRNA cleavage and poly(A) tail addition, CPSF2 influences transcript maturation, mRNA stability, and translation efficiency, thereby shaping global gene-expression programs. CPSF2-dependent 3′ end processing also interfaces with transcription termination and alternative polyadenylation, processes that remodel the transcriptome during proliferation and differentiation. Dysregulation of 3′ end formation and polyadenylation-site selection is frequently observed in disease-associated expression states, making CPSF2 a useful node for investigating RNA-processing phenotypes and their downstream cellular effects.
CPSF2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CPSF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CPSF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CPSF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CPSF2 protein expression.
This CRISPR knockout system enables efficient generation of CPSF2-deficient cell models for investigation of CPSF2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CPSF2 exon(s) critical for CPSF2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CPSF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CPSF2 CRISPR/Cas9 KO Plasmid (h) and CPSF2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CPSF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CPSF2 HDR Plasmid (h) and CPSF2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CPSF2 homology arms to support homology-directed repair at defined CPSF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.