CPSF1 Antibody (G-10): sc-166281

CPSF1 Antibody (G-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects CPSF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPSF1 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPSF1 plays a crucial role in the processing of the 3′ end of mRNA, which is essential for stability and translation of mRNA molecules. CPSF1 functions as part of the cleavage and polyadenylation specificity factor (CPSF) complex, which is vital for both cleavage of mRNA precursors and addition of poly(A) tails. CPSF1 is localized in the nucleoplasm, where CPSF1 interacts with other proteins to recognize AAUAAA signal in pre-mRNA, facilitating RNA cleavage and poly(A) synthesis. The largest subunit of CPSF can bind preferentially to AAUAAA-containing RNAs and interacts specifically with suppressor of forked subunit of cleavage stimulatory factor (CstF) and poly(A) polymerase, enhancing polyadenylation efficiency. CPSF1′s interaction with U1 snRNP-A protein (U1A) stabilizes binding to AAUAAA-containing RNAs, further increasing polyadenylation efficiency. CPSF1′s ability to interact with upstream elements of viral RNAs, such as those from HIV-1, underscores CPSF1′s importance in both cellular and viral mRNA processing, making CPSF1 a critical target for research in gene expression regulation and viral pathogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPSF1 Antibody (G-10) References:

1. CPSF1 mutations are associated with early-onset high myopia and involved in retinal ganglion cell axon projection.  |  Ouyang, J., et al. 2019. Hum Mol Genet. 28: 1959-1970. PMID: 30689892
2. CPSF1 mediates retinal vascular dysfunction in diabetes mellitus via the MAPK/ERK pathway.  |  Zhang, J., et al. 2022. Arch Physiol Biochem. 128: 708-715. PMID: 32046510
3. Aberrant expression of CPSF1 promotes head and neck squamous cell carcinoma via regulating alternative splicing.  |  Sakai, A., et al. 2020. PLoS One. 15: e0233380. PMID: 32437477
4. m6A-induced repression of SIAH1 facilitates alternative splicing of androgen receptor variant 7 by regulating CPSF1.  |  Xia, L., et al. 2022. Mol Ther Nucleic Acids. 28: 219-230. PMID: 35402071
5. CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression.  |  Kang, W., et al. 2022. Am J Cancer Res. 12: 4566-4583. PMID: 36381317
6. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.  |  Murthy, KG. and Manley, JL. 1995. Genes Dev. 9: 2672-83. PMID: 7590244
7. Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor.  |  Jenny, A. and Keller, W. 1995. Nucleic Acids Res. 23: 2629-35. PMID: 7651824
8. Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro.  |  Lutz, CS., et al. 1996. Genes Dev. 10: 325-37. PMID: 8595883
9. The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.  |  Barabino, SM., et al. 1997. Genes Dev. 11: 1703-16. PMID: 9224719
10. Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF.  |  Salinas, CA., et al. 1998. Mol Gen Genet. 257: 672-80. PMID: 9604891

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPSF1 Antibody (G-10) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CPSF1 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPSF1 Antibody (G-10) Specifications

Alternate Product Name: CPSF1

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: CPSF1

Epitope Species: human

Product Reactivity/Detection: CPSF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CPSF1 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin