Anti-CPSF1 Antibody (G-10) is a mouse monoclonal IgG2b (kappa light chain) CPSF1 antibody provided at 200 µg/ml
raised against amino acids 1-300 mapping at the N-terminus of CPSF1 of human origin
Anti-CPSF1 Antibody (G-10) is recommended for detection of CPSF1 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-CPSF1 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CPSF1 (G-10): sc-166281.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CPSF1 Antibody (G-10) is a high quality monoclonal CPSF1 antibody (also designated CPSF1 antibody) suitable for the detection of the CPSF1 protein of mouse, rat and human origin. CPSF1 Antibody (G-10) is available as both the non-conjugated anti-CPSF1 antibody form, as well as multiple conjugated forms of anti-CPSF1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Processing of the 3′ end of mRNA depends on several protein factors, one of which is the cleavage and polyadenylation specificity factor (CPSF). CPSF is required for both the cleavage of the mRNA precursor and for polyadenylation. CPSF, a multisubunit factor consisting of four subunits, is localized to the nucleoplasm and is excluded from cytoplasmic and nucleolar structures in HeLa cells. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. The largest subunit of CPSF can, by itself, bind preferentially to AAUAAA-containing RNAs and binds specifically to both the suppressor of forked subunit of the cleavage stimulatory factor (CstF) and to poly (A) polymerase. U1 snRNP-A protein (U1A) interacts with and affects the activity of CPSF by stabilizing the interaction of CPSF with the AAUAAA-containing RNAs to increase the efficiency of polyadenylation. Efficient processing of 3′ core poly(A) site also requires sequences 76 nucleotides upstream of the AAUAA hexamer. The largest subunit is able to interact directly with the HIV-1 upstream element to direct a stable binding of CPSF to the pre-mRNA and enhance the efficiency of polyadenylation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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