CPSF1 Antibody (B-5): sc-166282
- CPSF1 Antibody (B-5) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of CPSF1 of human origin
- recommended for detection of CPSF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for CPSF1 Antibody (B-5) for WB applications. This reagent is now offered in a bundle with CPSF1 Antibody (B-5) (see ordering information below).
CPSF1 Antibody (B-5) is a mouse monoclonal IgG2a antibody that detects CPSF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CPSF1, or cleavage and polyadenylation specificity factor 1, plays a crucial role in the processing of the 3′ end of mRNA, which is essential for the stability and translation of mRNA molecules. CPSF1 is primarily localized in the nucleoplasm, where CPSF1 is involved in recognizing the AAUAAA signal in pre-mRNA, facilitating both the cleavage of the mRNA precursor and the addition of the poly(A) tail. The structure of CPSF1 consists of multiple subunits that work together to ensure efficient mRNA processing; the largest subunit can bind preferentially to AAUAAA-containing RNAs and interacts with other key proteins, such as the cleavage stimulatory factor and poly(A) polymerase, to enhance the polyadenylation process. CPSF1′s interaction with U1 snRNP-A protein stabilizes binding to pre-mRNA, further increasing the efficiency of polyadenylation, which is vital for proper gene expression and regulation. anti-CPSF1 antibody (B-5) serves as an invaluable tool for researchers studying mRNA processing and its implications in various biological processes and diseases.
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CPSF1 Antibody (B-5) References:
- Cleavage and polyadenylation specificity factor 1 (CPSF1) regulates alternative splicing of interleukin 7 receptor (IL7R) exon 6. | Evsyukova, I., et al. 2013. RNA. 19: 103-15. PMID: 23151878
- Nuclear eIF4E Stimulates 3'-End Cleavage of Target RNAs. | Davis, MR., et al. 2019. Cell Rep. 27: 1397-1408.e4. PMID: 31042468
- mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1. | Du, Y., et al. 2020. Nat Commun. 11: 2449. PMID: 32415096
- Dissecting the heterogeneity of the alternative polyadenylation profiles in triple-negative breast cancers. | Wang, L., et al. 2020. Theranostics. 10: 10531-10547. PMID: 32929364
- CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors. | Guiro, J., et al. 2022. Biomolecules. 12: PMID: 35625631
- An Alternatively Spliced p62 Isoform Confers Resistance to Chemotherapy in Breast Cancer. | Guo, Q., et al. 2022. Cancer Res. 82: 4001-4015. PMID: 36041072
- Integrated RNA expression and alternative polyadenylation analysis identified CPSF1-CCDC137 oncogenic axis in lung adenocarcinoma. | Xudong, X., et al. 2024. Environ Toxicol. 39: 2405-2416. PMID: 38174951
- Alternative Polyadenylation Regulatory Factors Signature for Survival Prediction in Kidney Renal Cell Carcinoma. | Wang, X., et al. 2024. Cancer Inform. 23: 11769351231180789. PMID: 38617569
- Cleavage and polyadenylation machinery as a novel targetable vulnerability for human cancer. | Pagani, G. and Gandellini, P. 2024. Cancer Gene Ther. 31: 957-960. PMID: 38632357
- RETRACTION: Integrated RNA Expression and Alternative Polyadenylation Analysis Identified CPSF1-CCDC137 Oncogenic Axis in Lung Adenocarcinoma. | . 2025. Environ Toxicol.. PMID: 39838867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPSF1 Antibody (B-5) | sc-166282 | 200 µg/ml | $322.00 | |||
CPSF1 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-540067 | 200 µg Ab; 10 µg BP | $361.00 |