CPS1 Antibody (B-1): sc-376190

CPS1 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CPS1 Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPS1 (B-1) antibody targets a protein that plays a crucial role in the urea cycle, catalyzing the conversion of ammonia and bicarbonate into carbamoyl phosphate, which is essential for ammonia detoxification in the liver. This process maintains nitrogen balance in the body, and any CPS1 deficiency can lead to severe hyperammonemia, a condition causing neurological damage and inherited in an autosomal recessive manner. CPS1 (B-1) monoclonal antibody recognizes a protein primarily localized in liver cell mitochondria, where CPS1 functions under various metabolic signals, ensuring efficient ammonia processing during high protein intake or metabolic stress. CPS1′s ability to respond to cellular environment changes underscores metabolic homeostasis importance and highlights anti-CPS1 antibody (B-1) relevance in research focused on metabolic disorders and liver function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPS1 Antibody (B-1) References:

1. Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (cad).  |  Hewagama, A., et al. 1999. J Biol Chem. 274: 28240-5. PMID: 10497179
2. Regulation of carbamoyl phosphate synthetase by MAP kinase.  |  Graves, LM., et al. 2000. Nature. 403: 328-32. PMID: 10659854
3. Localization of the multifunctional protein CAD in astrocytes of rodent brain.  |  Cammer, W. and Downing, M. 1991. J Histochem Cytochem. 39: 695-700. PMID: 1673139
4. Cloning and sequence of a cDNA encoding human carbamyl phosphate synthetase I: molecular analysis of hyperammonemia.  |  Haraguchi, Y., et al. 1991. Gene. 107: 335-40. PMID: 1840546
5. CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells.  |  Kim, J., et al. 2017. Nature. 546: 168-172. PMID: 28538732
6. O-GlcNAcylation enhances CPS1 catalytic efficiency for ammonia and promotes ureagenesis.  |  Soria, LR., et al. 2022. Nat Commun. 13: 5212. PMID: 36064721
7. Unraveling the therapeutic potential of carbamoyl phosphate synthetase 1 (CPS1) in human diseases.  |  Zhang, L., et al. 2023. Bioorg Chem. 130: 106253. PMID: 36356370
8. NAGS, CPS1, and SLC25A13 (Citrin) at the Crossroads of Arginine and Pyrimidines Metabolism in Tumor Cells.  |  Owusu-Ansah, M., et al. 2023. Int J Mol Sci. 24: PMID: 37047726
9. Gasdermin-E-Dependent Non-Canonical Pyroptosis Promotes Drug-Induced Liver Failure by Promoting CPS1 deISGylation and Degradation.  |  Ouyang, SX., et al. 2024. Adv Sci (Weinh). 11: e2305715. PMID: 38417117
10. Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis.  |  Carrey, EA., et al. 1985. EMBO J. 4: 3735-42. PMID: 4092695
11. Phosphorylation and dephosphorylation of carbamoyl-phosphate synthetase II complex of rat ascites hepatoma cells.  |  Otsuki, T., et al. 1981. J Biochem. 89: 1367-74. PMID: 6115855
12. Mice deficient in the urea-cycle enzyme, carbamoyl phosphate synthetase I, die during the early neonatal period from hyperammonemia.  |  Schofield, JP., et al. 1999. Hepatology. 29: 181-5. PMID: 9862865

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPS1 Antibody (B-1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CPS1 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPS1 Antibody (B-1) Specifications

Alternate Product Name: CPS1

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1361-1500

Epitope Target: CPS1

Epitope Species: human

Product Reactivity/Detection: CPS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CPS1 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin