COX5a Antibody (A-5): sc-376907
- COX5a Antibody (A-5) is a mouse monoclonal IgG1 κ COX5a antibody, cited in 12 publications, provided at 200 µg/ml
- raised against amino acids 1-150 representing full length COX5a of human origin
- COX5a Antibody (A-5) is recommended for detection of cytochrome c oxidase Va of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- Anti-COX5a Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for COX5a Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with COX5a Antibody (A-5) (see ordering information below).
COX5a Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects COX5a of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-COX5a antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COX5a is a crucial component of the cytochrome c oxidase (COX) complex, which plays a vital role in cellular respiration by facilitating the final step of the electron transport chain. This process is essential for ATP production, as COX5a drives the creation of a proton gradient across the inner mitochondrial membrane, ultimately leading to ATP synthesis and water production from molecular oxygen and hydrogen ions. COX5a, as one of the two subunits of COX5, contains heme A and enables efficient electron transfer and energy production. COX5a expression responds to cellular oxygen levels - increasing during oxygen abundance to enhance aerobic respiration and decreasing under hypoxic conditions to conserve oxygen, highlighting COX5a′s role in maintaining cellular energy homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
COX5a Antibody (A-5) References:
- Amino acid replacement is rapid in primates for the mature polypeptides of COX subunits, but not for their targeting presequences. | Schmidt, TR., et al. 2002. Gene. 286: 13-9. PMID: 11943455
- Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1, or SURF1. | Williams, SL., et al. 2004. J Biol Chem. 279: 7462-9. PMID: 14607829
- Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1. | Stiburek, L., et al. 2005. Biochem J. 392: 625-32. PMID: 16083427
- Gene expression of cox5a, 5b, or 6b1 and their roles in preimplantation mouse embryos. | Cui, XS., et al. 2006. Biol Reprod. 74: 601-10. PMID: 16291927
- Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery. | De Angelis, PM., et al. 2006. Mol Cancer. 5: 20. PMID: 16709241
- HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. | Fukuda, R., et al. 2007. Cell. 129: 111-22. PMID: 17418790
- The isoforms of yeast cytochrome c oxidase subunit V alter the in vivo kinetic properties of the holoenzyme. | Waterland, RA., et al. 1991. J Biol Chem. 266: 4180-6. PMID: 1847916
- Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase. | Cumsky, MG., et al. 1985. Proc Natl Acad Sci U S A. 82: 2235-9. PMID: 2986105
- Overexpression of COX5A protects H9c2 cells against doxorubicin-induced cardiotoxicity. | Zhang, P., et al. 2020. Biochem Biophys Res Commun. 524: 43-49. PMID: 31980176
- COX5A Alleviates Doxorubicin-Induced Cardiotoxicity by Suppressing Oxidative Stress, Mitochondrial Dysfunction and Cardiomyocyte Apoptosis. | Zhang, P., et al. 2023. Int J Mol Sci. 24: PMID: 37373547
- COX5A as a potential biomarker for disease activity and organ damage in lupus. | Cai, M., et al. 2023. Clin Exp Med. 23: 4745-4756. PMID: 37891386
- Effects of oxygen concentration on the expression of cytochrome c and cytochrome c oxidase genes in yeast. | Burke, PV., et al. 1997. J Biol Chem. 272: 14705-12. PMID: 9169434
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COX5a Antibody (A-5) | sc-376907 | 200 µg/ml | $316.00 | |||
COX5a Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-529949 | 200 µg Ab; 10 µg BP | $354.00 | |||
COX5a Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-523133 | 200 µg Ab, 40 µg BP | $354.00 | |||
COX5a Antibody (A-5) AC | sc-376907 AC | 500 µg/ml, 25% agarose | $416.00 | |||
COX5a Antibody (A-5) HRP | sc-376907 HRP | 200 µg/ml | $316.00 | |||
COX5a Antibody (A-5) FITC | sc-376907 FITC | 200 µg/ml | $330.00 | |||
COX5a Antibody (A-5) PE | sc-376907 PE | 200 µg/ml | $343.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 488 | sc-376907 AF488 | 200 µg/ml | $357.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 546 | sc-376907 AF546 | 200 µg/ml | $357.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 594 | sc-376907 AF594 | 200 µg/ml | $357.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 647 | sc-376907 AF647 | 200 µg/ml | $357.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 680 | sc-376907 AF680 | 200 µg/ml | $357.00 | |||
COX5a Antibody (A-5) Alexa Fluor® 790 | sc-376907 AF790 | 200 µg/ml | $357.00 |